<?xml version="1.0" encoding="US-ASCII"?>  
<!-- Generated by iC(tm) - CompSci Interactive Converter - http://www.compsciresources.com -->     
<!-- Created: Mon May 02 18:46:46 UTC 2016 -->  
<linkbase xmlns="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd" xmlns:xbrldt="http://xbrl.org/2005/xbrldt">  
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_ConsolidatedBalanceSheet" roleURI="http://www.euroseas.gr/role/ConsolidatedBalanceSheet"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_ConsolidatedBalanceSheet_Parentheticals" roleURI="http://www.euroseas.gr/role/ConsolidatedBalanceSheet_Parentheticals"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_ConsolidatedIncomeStatement" roleURI="http://www.euroseas.gr/role/ConsolidatedIncomeStatement"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_ConsolidatedIncomeStatement_Parentheticals" roleURI="http://www.euroseas.gr/role/ConsolidatedIncomeStatement_Parentheticals"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_ShareholdersEquityType2or3" roleURI="http://www.euroseas.gr/role/ShareholdersEquityType2or3"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_ShareholdersEquityType2or3_Parentheticals" roleURI="http://www.euroseas.gr/role/ShareholdersEquityType2or3_Parentheticals"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_ConsolidatedCashFlow" roleURI="http://www.euroseas.gr/role/ConsolidatedCashFlow"/> 
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note1BasisofPresentationandGeneralInformation" roleURI="http://www.euroseas.gr/role/Note1BasisofPresentationandGeneralInformation"/> 
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note2SignificantAccountingPolicies" roleURI="http://www.euroseas.gr/role/Note2SignificantAccountingPolicies"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note3Inventories" roleURI="http://www.euroseas.gr/role/Note3Inventories"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note4AdvancesforVesselsunderConstruction" roleURI="http://www.euroseas.gr/role/Note4AdvancesforVesselsunderConstruction"/> 
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note5VesselsNet" roleURI="http://www.euroseas.gr/role/Note5VesselsNet"/> 
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note6DeferredChargesNet" roleURI="http://www.euroseas.gr/role/Note6DeferredChargesNet"/>  
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note7AccruedExpenses" roleURI="http://www.euroseas.gr/role/Note7AccruedExpenses"/> 
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note8RelatedPartyTransactions" roleURI="http://www.euroseas.gr/role/Note8RelatedPartyTransactions"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note9LongtermDebt" roleURI="http://www.euroseas.gr/role/Note9LongtermDebt"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note10IncomeTaxes" roleURI="http://www.euroseas.gr/role/Note10IncomeTaxes"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note11CommitmentsandContingencies" roleURI="http://www.euroseas.gr/role/Note11CommitmentsandContingencies"/>  
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note12StockIncentivePlan" roleURI="http://www.euroseas.gr/role/Note12StockIncentivePlan"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note13EarningsLossPerShare" roleURI="http://www.euroseas.gr/role/Note13EarningsLossPerShare"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note14VoyageVesselOperatingExpensesandCommissions" roleURI="http://www.euroseas.gr/role/Note14VoyageVesselOperatingExpensesandCommissions"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note15FinancialInstruments" roleURI="http://www.euroseas.gr/role/Note15FinancialInstruments"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note16DerivativeFinancialInstruments" roleURI="http://www.euroseas.gr/role/Note16DerivativeFinancialInstruments"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note17InvestmentinJointVentureandOtherInvestment" roleURI="http://www.euroseas.gr/role/Note17InvestmentinJointVentureandOtherInvestment"/>  
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note18Preferredshares" roleURI="http://www.euroseas.gr/role/Note18Preferredshares"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note19CommonStock" roleURI="http://www.euroseas.gr/role/Note19CommonStock"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note20SubsequentEvents" roleURI="http://www.euroseas.gr/role/Note20SubsequentEvents"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_AccountingPoliciesByPolicy" roleURI="http://www.euroseas.gr/role/AccountingPoliciesByPolicy"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note1BasisofPresentationandGeneralInformationTables" roleURI="http://www.euroseas.gr/role/Note1BasisofPresentationandGeneralInformationTables"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note3InventoriesTables" roleURI="http://www.euroseas.gr/role/Note3InventoriesTables"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note5VesselsNetTables" roleURI="http://www.euroseas.gr/role/Note5VesselsNetTables"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note6DeferredChargesNetTables" roleURI="http://www.euroseas.gr/role/Note6DeferredChargesNetTables"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note7AccruedExpensesTables" roleURI="http://www.euroseas.gr/role/Note7AccruedExpensesTables"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note9LongtermDebtTables" roleURI="http://www.euroseas.gr/role/Note9LongtermDebtTables"/>  
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note12StockIncentivePlanTables" roleURI="http://www.euroseas.gr/role/Note12StockIncentivePlanTables"/> 
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note13EarningsLossPerShareTables" roleURI="http://www.euroseas.gr/role/Note13EarningsLossPerShareTables"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note14VoyageVesselOperatingExpensesandCommissionsTables" roleURI="http://www.euroseas.gr/role/Note14VoyageVesselOperatingExpensesandCommissionsTables"/> 
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note15FinancialInstrumentsTables" roleURI="http://www.euroseas.gr/role/Note15FinancialInstrumentsTables"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note16DerivativeFinancialInstrumentsTables" roleURI="http://www.euroseas.gr/role/Note16DerivativeFinancialInstrumentsTables"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note17InvestmentinJointVentureandOtherInvestmentTables" roleURI="http://www.euroseas.gr/role/Note17InvestmentinJointVentureandOtherInvestmentTables"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note18PreferredsharesTables" roleURI="http://www.euroseas.gr/role/Note18PreferredsharesTables"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_CharterersIndividuallyAccountedforMorethan10oftheCompanysVoyageandTimeCharterRevenuesTable" roleURI="http://www.euroseas.gr/role/CharterersIndividuallyAccountedforMorethan10oftheCompanysVoyageandTimeCharterRevenuesTable"/> 
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_SummaryofInventoriesTable" roleURI="http://www.euroseas.gr/role/SummaryofInventoriesTable"/> 
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_SummaryofVesselsTable" roleURI="http://www.euroseas.gr/role/SummaryofVesselsTable"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_SummaryofDeferredChargesTable" roleURI="http://www.euroseas.gr/role/SummaryofDeferredChargesTable"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_SummaryofAccruedExpensesTable" roleURI="http://www.euroseas.gr/role/SummaryofAccruedExpensesTable"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_SummaryofLongtermDebtTable" roleURI="http://www.euroseas.gr/role/SummaryofLongtermDebtTable"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_SummaryofFutureAnnualLoanRepaymentsforLongtermDebtTable" roleURI="http://www.euroseas.gr/role/SummaryofFutureAnnualLoanRepaymentsforLongtermDebtTable"/> 
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_SummaryoftheStatusoftheCompanysNonvestedSharesTable" roleURI="http://www.euroseas.gr/role/SummaryoftheStatusoftheCompanysNonvestedSharesTable"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_SummaryofBasicandDilutedLossperCommonShareTable" roleURI="http://www.euroseas.gr/role/SummaryofBasicandDilutedLossperCommonShareTable"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_SummaryofVoyageVesselOperatingExpensesandCommissionsTable" roleURI="http://www.euroseas.gr/role/SummaryofVoyageVesselOperatingExpensesandCommissionsTable"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_CommissionConsistedofCommissionsChargedTable" roleURI="http://www.euroseas.gr/role/CommissionConsistedofCommissionsChargedTable"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_FairValueofCompanysLiabilitiesTable" roleURI="http://www.euroseas.gr/role/FairValueofCompanysLiabilitiesTable"/> 
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_AssetMeasuredatFairValueonaNonrecurringBasisTable" roleURI="http://www.euroseas.gr/role/AssetMeasuredatFairValueonaNonrecurringBasisTable"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_QuantitativeInformationaboutLevel3FairValueMeasurementsTable" roleURI="http://www.euroseas.gr/role/QuantitativeInformationaboutLevel3FairValueMeasurementsTable"/> 
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_SummaryofDerivativeActivityTable" roleURI="http://www.euroseas.gr/role/SummaryofDerivativeActivityTable"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_DerivativeNotDesignatedasHedgingInstrumentsbyAccountTypeTable" roleURI="http://www.euroseas.gr/role/DerivativeNotDesignatedasHedgingInstrumentsbyAccountTypeTable"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_GainorLossonDerivativesNotDesignatedasHedgingInstrumentsTable" roleURI="http://www.euroseas.gr/role/GainorLossonDerivativesNotDesignatedasHedgingInstrumentsTable"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_SummarizedFinancialInformationfortheJointVentureTable" roleURI="http://www.euroseas.gr/role/SummarizedFinancialInformationfortheJointVentureTable"/>  
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_InvestmentinJointVentureTable" roleURI="http://www.euroseas.gr/role/InvestmentinJointVentureTable"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_DividendsSeriesBPreferredSharesTable" roleURI="http://www.euroseas.gr/role/DividendsSeriesBPreferredSharesTable"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note1BasisofPresentationandGeneralInformationDetails" roleURI="http://www.euroseas.gr/role/Note1BasisofPresentationandGeneralInformationDetails"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note2SignificantAccountingPoliciesDetails" roleURI="http://www.euroseas.gr/role/Note2SignificantAccountingPoliciesDetails"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note4AdvancesforVesselsunderConstructionDetails" roleURI="http://www.euroseas.gr/role/Note4AdvancesforVesselsunderConstructionDetails"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note5VesselsNetDetails" roleURI="http://www.euroseas.gr/role/Note5VesselsNetDetails"/>     
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note8RelatedPartyTransactionsDetails" roleURI="http://www.euroseas.gr/role/Note8RelatedPartyTransactionsDetails"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note9LongtermDebtDetails" roleURI="http://www.euroseas.gr/role/Note9LongtermDebtDetails"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note10IncomeTaxesDetails" roleURI="http://www.euroseas.gr/role/Note10IncomeTaxesDetails"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note11CommitmentsandContingenciesDetails" roleURI="http://www.euroseas.gr/role/Note11CommitmentsandContingenciesDetails"/> 
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note12StockIncentivePlanDetails" roleURI="http://www.euroseas.gr/role/Note12StockIncentivePlanDetails"/>   
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note13EarningsLossPerShareDetails" roleURI="http://www.euroseas.gr/role/Note13EarningsLossPerShareDetails"/>  
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note15FinancialInstrumentsDetails" roleURI="http://www.euroseas.gr/role/Note15FinancialInstrumentsDetails"/>  
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note16DerivativeFinancialInstrumentsDetails" roleURI="http://www.euroseas.gr/role/Note16DerivativeFinancialInstrumentsDetails"/>  
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note17InvestmentinJointVentureandOtherInvestmentDetails" roleURI="http://www.euroseas.gr/role/Note17InvestmentinJointVentureandOtherInvestmentDetails"/> 
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note18PreferredsharesDetails" roleURI="http://www.euroseas.gr/role/Note18PreferredsharesDetails"/>  
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note19CommonStockDetails" roleURI="http://www.euroseas.gr/role/Note19CommonStockDetails"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#esea_r_Note20SubsequentEventsDetails" roleURI="http://www.euroseas.gr/role/Note20SubsequentEventsDetails"/>    
  <roleRef xlink:type="simple" xlink:href="esea-20151231.xsd#DocumentAndEntityInformation" roleURI="http://www.euroseas.gr/role/DocumentAndEntityInformation"/>     
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default" arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default"/> 
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain" arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain"/>    
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension" arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension"/>     
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member" arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member"/>   
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all" arcroleURI="http://xbrl.org/int/dim/arcrole/all"/>   
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/ConsolidatedBalanceSheet">  
    <loc xlink:type="locator" xlink:label="us-gaap_StatementLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementLineItems"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_StatementTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementTable"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StatementTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_StatementScenarioAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementScenarioAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable" xlink:to="us-gaap_StatementScenarioAxis" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_AssetsAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AssetsAbstract"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_AssetsAbstract" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_AssetsCurrentAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AssetsCurrentAbstract"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_AssetsCurrentAbstract" order="2" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_CashAndCashEquivalentsAtCarryingValue" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsCurrentAbstract" xlink:to="us-gaap_CashAndCashEquivalentsAtCarryingValue" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_AccountsReceivableNetCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccountsReceivableNetCurrent"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsCurrentAbstract" xlink:to="us-gaap_AccountsReceivableNetCurrent" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_OtherReceivablesNetCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_OtherReceivablesNetCurrent"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsCurrentAbstract" xlink:to="us-gaap_OtherReceivablesNetCurrent" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_InventoryNet" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InventoryNet"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsCurrentAbstract" xlink:to="us-gaap_InventoryNet" order="3" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_RestrictedCashAndCashEquivalentsAtCarryingValue" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RestrictedCashAndCashEquivalentsAtCarryingValue"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsCurrentAbstract" xlink:to="us-gaap_RestrictedCashAndCashEquivalentsAtCarryingValue" order="4" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PrepaidExpenseCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PrepaidExpenseCurrent"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsCurrentAbstract" xlink:to="us-gaap_PrepaidExpenseCurrent" order="5" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_DisposalGroupIncludingDiscontinuedOperationPropertyPlantAndEquipmentCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DisposalGroupIncludingDiscontinuedOperationPropertyPlantAndEquipmentCurrent"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsCurrentAbstract" xlink:to="us-gaap_DisposalGroupIncludingDiscontinuedOperationPropertyPlantAndEquipmentCurrent" order="6" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_AssetsCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AssetsCurrent"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsCurrentAbstract" xlink:to="us-gaap_AssetsCurrent" order="7" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_FixedAssetsAbstract" xlink:href="esea-20151231.xsd#esea_FixedAssetsAbstract"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="esea_FixedAssetsAbstract" order="3" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentNet" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_FixedAssetsAbstract" xlink:to="us-gaap_PropertyPlantAndEquipmentNet" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_AdvancesForVesselAcquisition" xlink:href="esea-20151231.xsd#esea_AdvancesForVesselAcquisition"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_FixedAssetsAbstract" xlink:to="esea_AdvancesForVesselAcquisition" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_LongTermAssetsAbstract" xlink:href="esea-20151231.xsd#esea_LongTermAssetsAbstract"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="esea_LongTermAssetsAbstract" order="4" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_RestrictedCashAndCashEquivalentsNoncurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RestrictedCashAndCashEquivalentsNoncurrent"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_LongTermAssetsAbstract" xlink:to="us-gaap_RestrictedCashAndCashEquivalentsNoncurrent" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DeferredCosts" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DeferredCosts"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_LongTermAssetsAbstract" xlink:to="us-gaap_DeferredCosts" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_InvestmentsAndOtherNoncurrentAssets" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InvestmentsAndOtherNoncurrentAssets"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_LongTermAssetsAbstract" xlink:to="us-gaap_InvestmentsAndOtherNoncurrentAssets" order="2" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestments" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestments"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_LongTermAssetsAbstract" xlink:to="us-gaap_EquityMethodInvestments" order="3" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_AssetsNoncurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AssetsNoncurrent"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_LongTermAssetsAbstract" xlink:to="us-gaap_AssetsNoncurrent" order="4" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_Assets" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_Assets"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_Assets" order="5" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_LiabilitiesAndStockholdersEquityAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LiabilitiesAndStockholdersEquityAbstract"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_LiabilitiesAndStockholdersEquityAbstract" order="6" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_LiabilitiesCurrentAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LiabilitiesCurrentAbstract"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_LiabilitiesCurrentAbstract" order="7" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_LongTermDebtCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LongTermDebtCurrent"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract" xlink:to="us-gaap_LongTermDebtCurrent" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract" xlink:to="us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_AccruedLiabilitiesCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccruedLiabilitiesCurrent"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract" xlink:to="us-gaap_AccruedLiabilitiesCurrent" order="2" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_LiabilitiesOfDisposalGroupIncludingDiscontinuedOperationCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LiabilitiesOfDisposalGroupIncludingDiscontinuedOperationCurrent"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract" xlink:to="us-gaap_LiabilitiesOfDisposalGroupIncludingDiscontinuedOperationCurrent" order="3" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DeferredRevenueCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DeferredRevenueCurrent"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract" xlink:to="us-gaap_DeferredRevenueCurrent" order="4" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_DueToRelatedPartiesCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DueToRelatedPartiesCurrent"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract" xlink:to="us-gaap_DueToRelatedPartiesCurrent" order="5" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeLiabilitiesCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeLiabilitiesCurrent"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract" xlink:to="us-gaap_DerivativeLiabilitiesCurrent" order="6" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_LiabilitiesCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LiabilitiesCurrent"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract" xlink:to="us-gaap_LiabilitiesCurrent" order="7" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_LiabilitiesNoncurrentAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LiabilitiesNoncurrentAbstract"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_LiabilitiesNoncurrentAbstract" order="8" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_LongTermDebtNoncurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LongTermDebtNoncurrent"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesNoncurrentAbstract" xlink:to="us-gaap_LongTermDebtNoncurrent" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeLiabilitiesNoncurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeLiabilitiesNoncurrent"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesNoncurrentAbstract" xlink:to="us-gaap_DerivativeLiabilitiesNoncurrent" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_LiabilitiesNoncurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LiabilitiesNoncurrent"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesNoncurrentAbstract" xlink:to="us-gaap_LiabilitiesNoncurrent" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_Liabilities" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_Liabilities"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_Liabilities" order="9" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_MezzanineEquityAbstract" xlink:href="esea-20151231.xsd#esea_MezzanineEquityAbstract"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="esea_MezzanineEquityAbstract" order="10" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PreferredStockValue" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockValue"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_PreferredStockValue" order="11" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_StockholdersEquityAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockholdersEquityAbstract"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StockholdersEquityAbstract" order="12" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_CommonStockValue" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CommonStockValue"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_CommonStockValue" order="13" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_AdditionalPaidInCapitalCommonStock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AdditionalPaidInCapitalCommonStock"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_AdditionalPaidInCapitalCommonStock" order="14" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_RetainedEarningsAccumulatedDeficit" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_RetainedEarningsAccumulatedDeficit" order="15" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_StockholdersEquity" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockholdersEquity"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StockholdersEquity" order="16" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_LiabilitiesAndStockholdersEquity" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_LiabilitiesAndStockholdersEquity" order="17" xbrldt:closed="true"/>    
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/ConsolidatedBalanceSheet_Parentheticals">  
    <loc xlink:type="locator" xlink:label="us-gaap_StatementLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementLineItems"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_StatementTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementTable"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StatementTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_StatementScenarioAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementScenarioAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable" xlink:to="us-gaap_StatementScenarioAxis" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_PreferredStockParOrStatedValuePerShare" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockParOrStatedValuePerShare"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_PreferredStockParOrStatedValuePerShare" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PreferredStockSharesAuthorized" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockSharesAuthorized"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_PreferredStockSharesAuthorized" order="2" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PreferredStockSharesIssued" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockSharesIssued"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_PreferredStockSharesIssued" order="3" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PreferredStockSharesOutstanding" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockSharesOutstanding"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_PreferredStockSharesOutstanding" order="4" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_CommonStockParOrStatedValuePerShare" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CommonStockParOrStatedValuePerShare"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_CommonStockParOrStatedValuePerShare" order="5" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_CommonStockSharesAuthorized" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CommonStockSharesAuthorized"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_CommonStockSharesAuthorized" order="6" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_CommonStockSharesIssued" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CommonStockSharesIssued"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_CommonStockSharesIssued" order="7" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_CommonStockSharesOutstanding" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CommonStockSharesOutstanding"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_CommonStockSharesOutstanding" order="8" xbrldt:closed="true"/>    
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/ConsolidatedIncomeStatement">     
    <loc xlink:type="locator" xlink:label="us-gaap_StatementLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementLineItems"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_StatementTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementTable"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StatementTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_StatementScenarioAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementScenarioAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable" xlink:to="us-gaap_StatementScenarioAxis" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_RevenuesAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RevenuesAbstract"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_RevenuesAbstract" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_VoyageRevenues" xlink:href="esea-20151231.xsd#esea_VoyageRevenues"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RevenuesAbstract" xlink:to="esea_VoyageRevenues" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_RevenueFromRelatedParties" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RevenueFromRelatedParties"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RevenuesAbstract" xlink:to="us-gaap_RevenueFromRelatedParties" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_SalesCommissionsAndFees" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SalesCommissionsAndFees"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RevenuesAbstract" xlink:to="us-gaap_SalesCommissionsAndFees" order="2" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_SalesRevenueNet" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SalesRevenueNet"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_SalesRevenueNet" order="2" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_OperatingExpensesAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_OperatingExpensesAbstract"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_OperatingExpensesAbstract" order="3" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_VoyageExpenses" xlink:href="esea-20151231.xsd#esea_VoyageExpenses"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OperatingExpensesAbstract" xlink:to="esea_VoyageExpenses" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_OperatingCostsAndExpenses" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_OperatingCostsAndExpenses"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OperatingExpensesAbstract" xlink:to="us-gaap_OperatingCostsAndExpenses" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_CostOfPropertyRepairsAndMaintenance" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CostOfPropertyRepairsAndMaintenance"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OperatingExpensesAbstract" xlink:to="us-gaap_CostOfPropertyRepairsAndMaintenance" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_Depreciation" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_Depreciation"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OperatingExpensesAbstract" xlink:to="us-gaap_Depreciation" order="3" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ProfessionalAndContractServicesExpense" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ProfessionalAndContractServicesExpense"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OperatingExpensesAbstract" xlink:to="us-gaap_ProfessionalAndContractServicesExpense" order="4" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_GeneralAndAdministrativeExpense" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_GeneralAndAdministrativeExpense"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OperatingExpensesAbstract" xlink:to="us-gaap_GeneralAndAdministrativeExpense" order="5" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_GainLossOnSaleOfPropertyPlantEquipment" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_GainLossOnSaleOfPropertyPlantEquipment"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OperatingExpensesAbstract" xlink:to="us-gaap_GainLossOnSaleOfPropertyPlantEquipment" order="6" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_AssetImpairmentCharges" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AssetImpairmentCharges"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OperatingExpensesAbstract" xlink:to="us-gaap_AssetImpairmentCharges" order="7" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_OperatingExpenses" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_OperatingExpenses"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_OperatingExpenses" order="4" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_OperatingIncomeLoss" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_OperatingIncomeLoss"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_OperatingIncomeLoss" order="5" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_OtherIncomeExpensesAbstract" xlink:href="esea-20151231.xsd#esea_OtherIncomeExpensesAbstract"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="esea_OtherIncomeExpensesAbstract" order="6" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_InterestAndDebtExpense" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InterestAndDebtExpense"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_OtherIncomeExpensesAbstract" xlink:to="us-gaap_InterestAndDebtExpense" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInstrumentsNotDesignatedAsHedgingInstrumentsGainLossNet" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInstrumentsNotDesignatedAsHedgingInstrumentsGainLossNet"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_OtherIncomeExpensesAbstract" xlink:to="us-gaap_DerivativeInstrumentsNotDesignatedAsHedgingInstrumentsGainLossNet" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ForeignCurrencyTransactionGainLossRealized" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ForeignCurrencyTransactionGainLossRealized"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_OtherIncomeExpensesAbstract" xlink:to="us-gaap_ForeignCurrencyTransactionGainLossRealized" order="2" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_InvestmentIncomeNet" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InvestmentIncomeNet"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_OtherIncomeExpensesAbstract" xlink:to="us-gaap_InvestmentIncomeNet" order="3" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_InterestAndOtherIncome" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InterestAndOtherIncome"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_OtherIncomeExpensesAbstract" xlink:to="us-gaap_InterestAndOtherIncome" order="4" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_NonoperatingIncomeExpense" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NonoperatingIncomeExpense"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_NonoperatingIncomeExpense" order="7" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_IncomeLossFromEquityMethodInvestments" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncomeLossFromEquityMethodInvestments"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NonoperatingIncomeExpense" xlink:to="us-gaap_IncomeLossFromEquityMethodInvestments" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_NetIncomeLoss" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetIncomeLoss"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_NetIncomeLoss" order="8" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PreferredStockDividendsIncomeStatementImpact" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockDividendsIncomeStatementImpact"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetIncomeLoss" xlink:to="us-gaap_PreferredStockDividendsIncomeStatementImpact" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic" order="9" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_EarningsPerShareBasicAndDiluted" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EarningsPerShareBasicAndDiluted"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_EarningsPerShareBasicAndDiluted" order="10" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_WeightedAverageNumberOfShareOutstandingBasicAndDiluted" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_WeightedAverageNumberOfShareOutstandingBasicAndDiluted"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_WeightedAverageNumberOfShareOutstandingBasicAndDiluted" order="11" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/ConsolidatedIncomeStatement_Parentheticals">     
    <loc xlink:type="locator" xlink:label="us-gaap_StatementLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementLineItems"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_StatementTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementTable"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StatementTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_StatementScenarioAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementScenarioAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable" xlink:to="us-gaap_StatementScenarioAxis" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_CommissionsRelatedParty" xlink:href="esea-20151231.xsd#esea_CommissionsRelatedParty"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="esea_CommissionsRelatedParty" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_VesselOperatingExpensesRelatedpary" xlink:href="esea-20151231.xsd#esea_VesselOperatingExpensesRelatedpary"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="esea_VesselOperatingExpensesRelatedpary" order="2" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_RelatedPartyTransactionSellingGeneralAndAdministrativeExpensesFromTransactionsWithRelatedParty" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RelatedPartyTransactionSellingGeneralAndAdministrativeExpensesFromTransactionsWithRelatedParty"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_RelatedPartyTransactionSellingGeneralAndAdministrativeExpensesFromTransactionsWithRelatedParty" order="3" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_NetLossOnSaleOfVesselsRelatedParty" xlink:href="esea-20151231.xsd#esea_NetLossOnSaleOfVesselsRelatedParty"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="esea_NetLossOnSaleOfVesselsRelatedParty" order="4" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/ShareholdersEquityType2or3">    
    <loc xlink:type="locator" xlink:label="us-gaap_StatementLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementLineItems"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_StatementTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementTable"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StatementTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_SubsidiarySaleOfStockAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable" xlink:to="us-gaap_SubsidiarySaleOfStockAxis" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_SaleOfStockNameOfTransactionDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_SubsidiarySaleOfStockAxis" xlink:to="us-gaap_SaleOfStockNameOfTransactionDomain" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_SaleOfStockNameOfTransactionDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_SubsidiarySaleOfStockAxis" xlink:to="us-gaap_SaleOfStockNameOfTransactionDomain_0" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_StatementEquityComponentsAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable" xlink:to="us-gaap_StatementEquityComponentsAxis" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_EquityComponentDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityComponentDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementEquityComponentsAxis" xlink:to="us-gaap_EquityComponentDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_EquityComponentDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityComponentDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementEquityComponentsAxis" xlink:to="us-gaap_EquityComponentDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_CommonStockMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CommonStockMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain" xlink:to="us-gaap_CommonStockMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_AdditionalPaidInCapitalMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AdditionalPaidInCapitalMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain" xlink:to="us-gaap_AdditionalPaidInCapitalMember" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_RetainedEarningsMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RetainedEarningsMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain" xlink:to="us-gaap_RetainedEarningsMember" order="2" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PrivatePlacementMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PrivatePlacementMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SaleOfStockNameOfTransactionDomain" xlink:to="us-gaap_PrivatePlacementMember" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_RightsOfferingMember" xlink:href="esea-20151231.xsd#esea_RightsOfferingMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SaleOfStockNameOfTransactionDomain" xlink:to="esea_RightsOfferingMember" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_StockholdersEquity" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockholdersEquity"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StockholdersEquity" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_SharesOutstanding" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SharesOutstanding"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_SharesOutstanding" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic" order="3" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_StockIssuedDuringPeriodValueNewIssues" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueNewIssues"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StockIssuedDuringPeriodValueNewIssues" order="4" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_StockIssuedDuringPeriodSharesNewIssues" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StockIssuedDuringPeriodSharesNewIssues" order="5" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_NewSharesValueDueToRoundingReverseStockSplit" xlink:href="esea-20151231.xsd#esea_NewSharesValueDueToRoundingReverseStockSplit"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="esea_NewSharesValueDueToRoundingReverseStockSplit" order="6" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_NewSharesDueToRoundingReverseStockSplit" xlink:href="esea-20151231.xsd#esea_NewSharesDueToRoundingReverseStockSplit"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="esea_NewSharesDueToRoundingReverseStockSplit" order="7" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue" order="8" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures" order="9" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_StockRepurchasedAndRetiredDuringPeriodValue" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockRepurchasedAndRetiredDuringPeriodValue"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StockRepurchasedAndRetiredDuringPeriodValue" order="10" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_StockRepurchasedAndRetiredDuringPeriodShares" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockRepurchasedAndRetiredDuringPeriodShares"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StockRepurchasedAndRetiredDuringPeriodShares" order="11" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_Dividends" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_Dividends"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_Dividends" order="12" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/ShareholdersEquityType2or3_Parentheticals">    
    <loc xlink:type="locator" xlink:label="us-gaap_StatementLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementLineItems"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_StatementTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementTable"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StatementTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_SubsidiarySaleOfStockAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable" xlink:to="us-gaap_SubsidiarySaleOfStockAxis" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_SaleOfStockNameOfTransactionDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_SubsidiarySaleOfStockAxis" xlink:to="us-gaap_SaleOfStockNameOfTransactionDomain" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_SaleOfStockNameOfTransactionDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_SubsidiarySaleOfStockAxis" xlink:to="us-gaap_SaleOfStockNameOfTransactionDomain_0" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_StatementEquityComponentsAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable" xlink:to="us-gaap_StatementEquityComponentsAxis" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_EquityComponentDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityComponentDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementEquityComponentsAxis" xlink:to="us-gaap_EquityComponentDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_EquityComponentDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityComponentDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementEquityComponentsAxis" xlink:to="us-gaap_EquityComponentDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_CommonStockMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CommonStockMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain" xlink:to="us-gaap_CommonStockMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_AdditionalPaidInCapitalMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AdditionalPaidInCapitalMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain" xlink:to="us-gaap_AdditionalPaidInCapitalMember" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_RetainedEarningsMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RetainedEarningsMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain" xlink:to="us-gaap_RetainedEarningsMember" order="2" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PrivatePlacementMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PrivatePlacementMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SaleOfStockNameOfTransactionDomain" xlink:to="us-gaap_PrivatePlacementMember" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_RightsOfferingMember" xlink:href="esea-20151231.xsd#esea_RightsOfferingMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SaleOfStockNameOfTransactionDomain" xlink:to="esea_RightsOfferingMember" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_CommonStockDividendsPerShareDeclared" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CommonStockDividendsPerShareDeclared"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_CommonStockDividendsPerShareDeclared" order="1" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/ConsolidatedCashFlow">   
    <loc xlink:type="locator" xlink:label="us-gaap_StatementLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementLineItems"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_StatementTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementTable"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_StatementTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_StatementScenarioAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementScenarioAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable" xlink:to="us-gaap_StatementScenarioAxis" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_NetIncomeLoss" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetIncomeLoss"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_NetIncomeLoss" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_Depreciation" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_Depreciation"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_Depreciation" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_AssetImpairmentCharges" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AssetImpairmentCharges"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_AssetImpairmentCharges" order="3" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_AmortizationOfFinancingCosts" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AmortizationOfFinancingCosts"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_AmortizationOfFinancingCosts" order="4" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_GainLossOnSaleOfPropertyPlantEquipment" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_GainLossOnSaleOfPropertyPlantEquipment"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_GainLossOnSaleOfPropertyPlantEquipment" order="5" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_ShareBasedCompensation" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ShareBasedCompensation"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_ShareBasedCompensation" order="6" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_UnrealizedGainLossOnDerivatives" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_UnrealizedGainLossOnDerivatives"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_UnrealizedGainLossOnDerivatives" order="7" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_InvestmentIncomeDividend" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InvestmentIncomeDividend"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_InvestmentIncomeDividend" order="8" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_IncomeLossFromEquityMethodInvestments" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncomeLossFromEquityMethodInvestments"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_IncomeLossFromEquityMethodInvestments" order="9" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_IncreaseDecreaseInOperatingCapitalAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncreaseDecreaseInOperatingCapitalAbstract"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_IncreaseDecreaseInOperatingCapitalAbstract" order="10" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_IncreaseDecreaseInAbstract" xlink:href="esea-20151231.xsd#esea_IncreaseDecreaseInAbstract"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="esea_IncreaseDecreaseInAbstract" order="11" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_IncreaseDecreaseInAccountsReceivable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsReceivable"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_IncreaseDecreaseInAccountsReceivable" order="12" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_IncreaseDecreaseInPrepaidExpense" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncreaseDecreaseInPrepaidExpense"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_IncreaseDecreaseInPrepaidExpense" order="13" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables" order="14" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_IncreaseDecreaseInInventories" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncreaseDecreaseInInventories"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_IncreaseDecreaseInInventories" order="15" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_IncreaseDecreaseInDueFromAffiliatesCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncreaseDecreaseInDueFromAffiliatesCurrent"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_IncreaseDecreaseInDueFromAffiliatesCurrent" order="16" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_IncreaseDecreaseInAbstract0" xlink:href="esea-20151231.xsd#esea_IncreaseDecreaseInAbstract0"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="esea_IncreaseDecreaseInAbstract0" order="17" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_IncreaseDecreaseInDueToAffiliates" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncreaseDecreaseInDueToAffiliates"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_IncreaseDecreaseInDueToAffiliates" order="18" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_IncreaseDecreaseInAccountsPayableTrade" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsPayableTrade"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_IncreaseDecreaseInAccountsPayableTrade" order="19" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_IncreaseDecreaseInAccountsPayableAndAccruedLiabilities" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsPayableAndAccruedLiabilities"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_IncreaseDecreaseInAccountsPayableAndAccruedLiabilities" order="20" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_IncreaseDecreaseInDeferredRevenue" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncreaseDecreaseInDeferredRevenue"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:to="us-gaap_IncreaseDecreaseInDeferredRevenue" order="21" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_NetCashProvidedByUsedInOperatingActivities" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_NetCashProvidedByUsedInOperatingActivities" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract" order="3" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PaymentsForDeposits" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PaymentsForDeposits"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract" xlink:to="us-gaap_PaymentsForDeposits" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ProceedsFromSaleOfOtherPropertyPlantAndEquipment" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ProceedsFromSaleOfOtherPropertyPlantAndEquipment"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract" xlink:to="us-gaap_ProceedsFromSaleOfOtherPropertyPlantAndEquipment" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PaymentsToAcquireInterestInJointVenture" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PaymentsToAcquireInterestInJointVenture"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract" xlink:to="us-gaap_PaymentsToAcquireInterestInJointVenture" order="2" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PaymentsToAcquirePropertyPlantAndEquipment" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract" xlink:to="us-gaap_PaymentsToAcquirePropertyPlantAndEquipment" order="3" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_PaymentsToAcquireOtherInvestments" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PaymentsToAcquireOtherInvestments"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract" xlink:to="us-gaap_PaymentsToAcquireOtherInvestments" order="4" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_IncreaseDecreaseInRetentionAccounts" xlink:href="esea-20151231.xsd#esea_IncreaseDecreaseInRetentionAccounts"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract" xlink:to="esea_IncreaseDecreaseInRetentionAccounts" order="5" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_IncreaseDecreaseInRestrictedCash" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncreaseDecreaseInRestrictedCash"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract" xlink:to="us-gaap_IncreaseDecreaseInRestrictedCash" order="6" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract" xlink:to="us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment" order="7" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_NetCashProvidedByUsedInInvestingActivities" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_NetCashProvidedByUsedInInvestingActivities" order="4" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract" order="5" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract" xlink:to="us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ProceedsFromIssuanceOfCommonStock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ProceedsFromIssuanceOfCommonStock"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract" xlink:to="us-gaap_ProceedsFromIssuanceOfCommonStock" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PaymentsForRepurchaseOfCommonStock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PaymentsForRepurchaseOfCommonStock"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract" xlink:to="us-gaap_PaymentsForRepurchaseOfCommonStock" order="2" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PaymentsOfStockIssuanceCosts" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PaymentsOfStockIssuanceCosts"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract" xlink:to="us-gaap_PaymentsOfStockIssuanceCosts" order="3" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PaymentsOfDividends" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PaymentsOfDividends"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract" xlink:to="us-gaap_PaymentsOfDividends" order="4" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PaymentsOfFinancingCosts" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PaymentsOfFinancingCosts"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract" xlink:to="us-gaap_PaymentsOfFinancingCosts" order="5" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ProceedsFromIssuanceOfLongTermDebt" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ProceedsFromIssuanceOfLongTermDebt"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract" xlink:to="us-gaap_ProceedsFromIssuanceOfLongTermDebt" order="6" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_RepaymentsOfBankDebt" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RepaymentsOfBankDebt"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract" xlink:to="us-gaap_RepaymentsOfBankDebt" order="7" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_NetCashProvidedByUsedInFinancingActivities" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_NetCashProvidedByUsedInFinancingActivities" order="6" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInFinancingActivities" xlink:to="us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_CashAndCashEquivalentsAtCarryingValue" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NetCashProvidedByUsedInFinancingActivities" xlink:to="us-gaap_CashAndCashEquivalentsAtCarryingValue" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_SupplementalCashFlowInformationAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SupplementalCashFlowInformationAbstract"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="us-gaap_SupplementalCashFlowInformationAbstract" order="7" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_InterestPaidNet" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InterestPaidNet"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SupplementalCashFlowInformationAbstract" xlink:to="us-gaap_InterestPaidNet" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_FinancingAndInvestingActivitiesFeesAbstract" xlink:href="esea-20151231.xsd#esea_FinancingAndInvestingActivitiesFeesAbstract"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems" xlink:to="esea_FinancingAndInvestingActivitiesFeesAbstract" order="8" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_DebtIssuanceCostsIncurredDuringNoncashOrPartialNoncashTransaction" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtIssuanceCostsIncurredDuringNoncashOrPartialNoncashTransaction"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_FinancingAndInvestingActivitiesFeesAbstract" xlink:to="us-gaap_DebtIssuanceCostsIncurredDuringNoncashOrPartialNoncashTransaction" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_LiabilitiesAssumed1" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LiabilitiesAssumed1"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_FinancingAndInvestingActivitiesFeesAbstract" xlink:to="us-gaap_LiabilitiesAssumed1" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DividendsPaidinkind" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DividendsPaidinkind"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_FinancingAndInvestingActivitiesFeesAbstract" xlink:to="us-gaap_DividendsPaidinkind" order="2" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_CapitalExpendituresIncurredButNotYetPaid" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CapitalExpendituresIncurredButNotYetPaid"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_FinancingAndInvestingActivitiesFeesAbstract" xlink:to="us-gaap_CapitalExpendituresIncurredButNotYetPaid" order="3" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note1BasisofPresentationandGeneralInformation">   
    <loc xlink:type="locator" xlink:label="us-gaap_DisclosureTextBlockAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DisclosureTextBlockAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_BusinessDescriptionAndBasisOfPresentationTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_BusinessDescriptionAndBasisOfPresentationTextBlock"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisclosureTextBlockAbstract" xlink:to="us-gaap_BusinessDescriptionAndBasisOfPresentationTextBlock" order="0" xbrldt:closed="true"/>     
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note2SignificantAccountingPolicies">  
    <loc xlink:type="locator" xlink:label="us-gaap_AccountingPoliciesAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccountingPoliciesAbstract"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_SignificantAccountingPoliciesTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SignificantAccountingPoliciesTextBlock"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_SignificantAccountingPoliciesTextBlock" order="0" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note3Inventories">    
    <loc xlink:type="locator" xlink:label="us-gaap_InventoryDisclosureAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InventoryDisclosureAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_InventoryDisclosureTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InventoryDisclosureTextBlock"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InventoryDisclosureAbstract" xlink:to="us-gaap_InventoryDisclosureTextBlock" order="0" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note4AdvancesforVesselsunderConstruction">   
    <loc xlink:type="locator" xlink:label="esea_AdvancesForVesselsUnderConstructionAbstract" xlink:href="esea-20151231.xsd#esea_AdvancesForVesselsUnderConstructionAbstract"/>     
    <loc xlink:type="locator" xlink:label="esea_AdvancesForVesselsUnderConstructionTextBlock" xlink:href="esea-20151231.xsd#esea_AdvancesForVesselsUnderConstructionTextBlock"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_AdvancesForVesselsUnderConstructionAbstract" xlink:to="esea_AdvancesForVesselsUnderConstructionTextBlock" order="0" xbrldt:closed="true"/>    
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note5VesselsNet">   
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentAbstract"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentDisclosureTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentDisclosureTextBlock"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentAbstract" xlink:to="us-gaap_PropertyPlantAndEquipmentDisclosureTextBlock" order="0" xbrldt:closed="true"/>     
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note6DeferredChargesNet"> 
    <loc xlink:type="locator" xlink:label="esea_DeferredChargesAbstract" xlink:href="esea-20151231.xsd#esea_DeferredChargesAbstract"/>     
    <loc xlink:type="locator" xlink:label="esea_DeferredChargesTextBlock" xlink:href="esea-20151231.xsd#esea_DeferredChargesTextBlock"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_DeferredChargesAbstract" xlink:to="esea_DeferredChargesTextBlock" order="0" xbrldt:closed="true"/>    
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note7AccruedExpenses">   
    <loc xlink:type="locator" xlink:label="us-gaap_PayablesAndAccrualsAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PayablesAndAccrualsAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_AccountsPayableAndAccruedLiabilitiesDisclosureTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccountsPayableAndAccruedLiabilitiesDisclosureTextBlock"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PayablesAndAccrualsAbstract" xlink:to="us-gaap_AccountsPayableAndAccruedLiabilitiesDisclosureTextBlock" order="0" xbrldt:closed="true"/>     
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note8RelatedPartyTransactions">  
    <loc xlink:type="locator" xlink:label="us-gaap_RelatedPartyTransactionsAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RelatedPartyTransactionsAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_RelatedPartyTransactionsDisclosureTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RelatedPartyTransactionsDisclosureTextBlock"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyTransactionsAbstract" xlink:to="us-gaap_RelatedPartyTransactionsDisclosureTextBlock" order="0" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note9LongtermDebt">     
    <loc xlink:type="locator" xlink:label="us-gaap_DisclosureTextBlockAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DisclosureTextBlockAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_LongTermDebtTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LongTermDebtTextBlock"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisclosureTextBlockAbstract" xlink:to="us-gaap_LongTermDebtTextBlock" order="0" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note10IncomeTaxes">     
    <loc xlink:type="locator" xlink:label="us-gaap_IncomeTaxDisclosureAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncomeTaxDisclosureAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_IncomeTaxDisclosureTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncomeTaxDisclosureTextBlock"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_IncomeTaxDisclosureAbstract" xlink:to="us-gaap_IncomeTaxDisclosureTextBlock" order="0" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note11CommitmentsandContingencies"> 
    <loc xlink:type="locator" xlink:label="us-gaap_CommitmentsAndContingenciesDisclosureAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CommitmentsAndContingenciesDisclosureAbstract"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_CommitmentsAndContingenciesDisclosureTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CommitmentsAndContingenciesDisclosureTextBlock"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_CommitmentsAndContingenciesDisclosureAbstract" xlink:to="us-gaap_CommitmentsAndContingenciesDisclosureTextBlock" order="0" xbrldt:closed="true"/>    
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note12StockIncentivePlan">  
    <loc xlink:type="locator" xlink:label="us-gaap_DisclosureOfCompensationRelatedCostsSharebasedPaymentsAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DisclosureOfCompensationRelatedCostsSharebasedPaymentsAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_DisclosureOfCompensationRelatedCostsShareBasedPaymentsTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DisclosureOfCompensationRelatedCostsShareBasedPaymentsTextBlock"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisclosureOfCompensationRelatedCostsSharebasedPaymentsAbstract" xlink:to="us-gaap_DisclosureOfCompensationRelatedCostsShareBasedPaymentsTextBlock" order="0" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note13EarningsLossPerShare">    
    <loc xlink:type="locator" xlink:label="us-gaap_EarningsPerShareAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EarningsPerShareAbstract"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_EarningsPerShareTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EarningsPerShareTextBlock"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EarningsPerShareAbstract" xlink:to="us-gaap_EarningsPerShareTextBlock" order="0" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note14VoyageVesselOperatingExpensesandCommissions">  
    <loc xlink:type="locator" xlink:label="esea_VesselVoyageAndOperatingExpensesAbstract" xlink:href="esea-20151231.xsd#esea_VesselVoyageAndOperatingExpensesAbstract"/>    
    <loc xlink:type="locator" xlink:label="esea_VesselVoyageAndOperatingExpensesTextBlock" xlink:href="esea-20151231.xsd#esea_VesselVoyageAndOperatingExpensesTextBlock"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_VesselVoyageAndOperatingExpensesAbstract" xlink:to="esea_VesselVoyageAndOperatingExpensesTextBlock" order="0" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note15FinancialInstruments">    
    <loc xlink:type="locator" xlink:label="us-gaap_DisclosureTextBlockSupplementAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DisclosureTextBlockSupplementAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_FinancialInstrumentsDisclosureTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FinancialInstrumentsDisclosureTextBlock"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisclosureTextBlockSupplementAbstract" xlink:to="us-gaap_FinancialInstrumentsDisclosureTextBlock" order="0" xbrldt:closed="true"/>    
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note16DerivativeFinancialInstruments">    
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosureAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosureAbstract"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosureTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosureTextBlock"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosureAbstract" xlink:to="us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosureTextBlock" order="0" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note17InvestmentinJointVentureandOtherInvestment"> 
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestmentsAndJointVenturesAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestmentsAndJointVenturesAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestmentsDisclosureTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestmentsDisclosureTextBlock"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityMethodInvestmentsAndJointVenturesAbstract" xlink:to="us-gaap_EquityMethodInvestmentsDisclosureTextBlock" order="0" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note18Preferredshares">    
    <loc xlink:type="locator" xlink:label="us-gaap_DisclosureTextBlockSupplementAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DisclosureTextBlockSupplementAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_PreferredStockTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockTextBlock"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisclosureTextBlockSupplementAbstract" xlink:to="us-gaap_PreferredStockTextBlock" order="0" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note19CommonStock">     
    <loc xlink:type="locator" xlink:label="us-gaap_StockholdersEquityNoteAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockholdersEquityNoteAbstract"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_StockholdersEquityNoteDisclosureTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockholdersEquityNoteDisclosureTextBlock"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StockholdersEquityNoteAbstract" xlink:to="us-gaap_StockholdersEquityNoteDisclosureTextBlock" order="0" xbrldt:closed="true"/>    
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note20SubsequentEvents">     
    <loc xlink:type="locator" xlink:label="us-gaap_SubsequentEventsAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SubsequentEventsAbstract"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_SubsequentEventsTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SubsequentEventsTextBlock"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventsAbstract" xlink:to="us-gaap_SubsequentEventsTextBlock" order="0" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/AccountingPoliciesByPolicy">    
    <loc xlink:type="locator" xlink:label="us-gaap_AccountingPoliciesAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccountingPoliciesAbstract"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_ConsolidationPolicyTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ConsolidationPolicyTextBlock"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_ConsolidationPolicyTextBlock" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_UseOfEstimates" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_UseOfEstimates"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_UseOfEstimates" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ComprehensiveIncomePolicyPolicyTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ComprehensiveIncomePolicyPolicyTextBlock"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_ComprehensiveIncomePolicyPolicyTextBlock" order="2" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ForeignCurrencyTransactionsAndTranslationsPolicyTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ForeignCurrencyTransactionsAndTranslationsPolicyTextBlock"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_ForeignCurrencyTransactionsAndTranslationsPolicyTextBlock" order="3" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_CashAndCashEquivalentsPolicyTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CashAndCashEquivalentsPolicyTextBlock"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_CashAndCashEquivalentsPolicyTextBlock" order="4" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_CashAndCashEquivalentsRestrictedCashAndCashEquivalentsPolicy" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CashAndCashEquivalentsRestrictedCashAndCashEquivalentsPolicy"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_CashAndCashEquivalentsRestrictedCashAndCashEquivalentsPolicy" order="5" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_TradeAndOtherAccountsReceivablePolicy" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_TradeAndOtherAccountsReceivablePolicy"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_TradeAndOtherAccountsReceivablePolicy" order="6" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_InventoryPolicyTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InventoryPolicyTextBlock"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_InventoryPolicyTextBlock" order="7" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_VesselsPolicyTextBlock" xlink:href="esea-20151231.xsd#esea_VesselsPolicyTextBlock"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="esea_VesselsPolicyTextBlock" order="8" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_DepreciationDepletionAndAmortizationPolicyTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DepreciationDepletionAndAmortizationPolicyTextBlock"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_DepreciationDepletionAndAmortizationPolicyTextBlock" order="9" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_AssetsHeldForSalePolicyTextBlock" xlink:href="esea-20151231.xsd#esea_AssetsHeldForSalePolicyTextBlock"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="esea_AssetsHeldForSalePolicyTextBlock" order="10" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_InsurancePremiumsRevenueRecognitionPolicy" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InsurancePremiumsRevenueRecognitionPolicy"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_InsurancePremiumsRevenueRecognitionPolicy" order="11" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_RevenueRecognitionPolicyTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RevenueRecognitionPolicyTextBlock"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_RevenueRecognitionPolicyTextBlock" order="12" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_DrydockingAndSpecialSurveyExpensesPolicyTextBlock" xlink:href="esea-20151231.xsd#esea_DrydockingAndSpecialSurveyExpensesPolicyTextBlock"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="esea_DrydockingAndSpecialSurveyExpensesPolicyTextBlock" order="13" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PensionAndOtherPostretirementPlansPolicy" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PensionAndOtherPostretirementPlansPolicy"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_PensionAndOtherPostretirementPlansPolicy" order="14" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_FinanceLoansAndLeasesReceivablePolicy" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FinanceLoansAndLeasesReceivablePolicy"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_FinanceLoansAndLeasesReceivablePolicy" order="15" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueMeasurementPolicyPolicyTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueMeasurementPolicyPolicyTextBlock"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_FairValueMeasurementPolicyPolicyTextBlock" order="16" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_ShareBasedCompensationOptionAndIncentivePlansPolicy" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ShareBasedCompensationOptionAndIncentivePlansPolicy"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_ShareBasedCompensationOptionAndIncentivePlansPolicy" order="17" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestmentsPolicy" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestmentsPolicy"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_EquityMethodInvestmentsPolicy" order="18" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ImpairmentOrDisposalOfLongLivedAssetsIncludingIntangibleAssetsPolicyPolicyTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ImpairmentOrDisposalOfLongLivedAssetsIncludingIntangibleAssetsPolicyPolicyTextBlock"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_ImpairmentOrDisposalOfLongLivedAssetsIncludingIntangibleAssetsPolicyPolicyTextBlock" order="19" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_EquityAndCostMethodInvestmentsPolicy" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityAndCostMethodInvestmentsPolicy"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_EquityAndCostMethodInvestmentsPolicy" order="20" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativesPolicyTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativesPolicyTextBlock"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_DerivativesPolicyTextBlock" order="21" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_StockholdersEquityNoteRedeemablePreferredStockIssuePolicy" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockholdersEquityNoteRedeemablePreferredStockIssuePolicy"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_StockholdersEquityNoteRedeemablePreferredStockIssuePolicy" order="22" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_EarningsPerSharePolicyTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EarningsPerSharePolicyTextBlock"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_EarningsPerSharePolicyTextBlock" order="23" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_SegmentReportingPolicyPolicyTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SegmentReportingPolicyPolicyTextBlock"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_SegmentReportingPolicyPolicyTextBlock" order="24" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_NewAccountingPronouncementsPolicyPolicyTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NewAccountingPronouncementsPolicyPolicyTextBlock"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AccountingPoliciesAbstract" xlink:to="us-gaap_NewAccountingPronouncementsPolicyPolicyTextBlock" order="25" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note1BasisofPresentationandGeneralInformationTables">    
    <loc xlink:type="locator" xlink:label="us-gaap_DisclosureTextBlockAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DisclosureTextBlockAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_SchedulesOfConcentrationOfRiskByRiskFactorTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SchedulesOfConcentrationOfRiskByRiskFactorTextBlock"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisclosureTextBlockAbstract" xlink:to="us-gaap_SchedulesOfConcentrationOfRiskByRiskFactorTextBlock" order="0" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note3InventoriesTables">     
    <loc xlink:type="locator" xlink:label="us-gaap_InventoryDisclosureAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InventoryDisclosureAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ScheduleOfInventoryCurrentTableTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScheduleOfInventoryCurrentTableTextBlock"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InventoryDisclosureAbstract" xlink:to="us-gaap_ScheduleOfInventoryCurrentTableTextBlock" order="0" xbrldt:closed="true"/>     
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note5VesselsNetTables">    
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentAbstract"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTextBlock"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentAbstract" xlink:to="us-gaap_PropertyPlantAndEquipmentTextBlock" order="0" xbrldt:closed="true"/>     
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note6DeferredChargesNetTables">  
    <loc xlink:type="locator" xlink:label="esea_DeferredChargesAbstract" xlink:href="esea-20151231.xsd#esea_DeferredChargesAbstract"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_DeferredCostsCapitalizedPrepaidAndOtherAssetsDisclosureTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DeferredCostsCapitalizedPrepaidAndOtherAssetsDisclosureTextBlock"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_DeferredChargesAbstract" xlink:to="us-gaap_DeferredCostsCapitalizedPrepaidAndOtherAssetsDisclosureTextBlock" order="0" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note7AccruedExpensesTables">    
    <loc xlink:type="locator" xlink:label="us-gaap_PayablesAndAccrualsAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PayablesAndAccrualsAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ScheduleOfAccruedLiabilitiesTableTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScheduleOfAccruedLiabilitiesTableTextBlock"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PayablesAndAccrualsAbstract" xlink:to="us-gaap_ScheduleOfAccruedLiabilitiesTableTextBlock" order="0" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note9LongtermDebtTables"> 
    <loc xlink:type="locator" xlink:label="us-gaap_DisclosureTextBlockAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DisclosureTextBlockAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ScheduleOfDebtInstrumentsTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScheduleOfDebtInstrumentsTextBlock"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisclosureTextBlockAbstract" xlink:to="us-gaap_ScheduleOfDebtInstrumentsTextBlock" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_ScheduleOfFutureAnnualLoanRepaymentsTableTextBlock" xlink:href="esea-20151231.xsd#esea_ScheduleOfFutureAnnualLoanRepaymentsTableTextBlock"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisclosureTextBlockAbstract" xlink:to="esea_ScheduleOfFutureAnnualLoanRepaymentsTableTextBlock" order="1" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note12StockIncentivePlanTables">   
    <loc xlink:type="locator" xlink:label="us-gaap_DisclosureOfCompensationRelatedCostsSharebasedPaymentsAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DisclosureOfCompensationRelatedCostsSharebasedPaymentsAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ScheduleOfNonvestedShareActivityTableTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScheduleOfNonvestedShareActivityTableTextBlock"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisclosureOfCompensationRelatedCostsSharebasedPaymentsAbstract" xlink:to="us-gaap_ScheduleOfNonvestedShareActivityTableTextBlock" order="0" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note13EarningsLossPerShareTables">     
    <loc xlink:type="locator" xlink:label="us-gaap_EarningsPerShareAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EarningsPerShareAbstract"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ScheduleOfEarningsPerShareBasicAndDilutedTableTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScheduleOfEarningsPerShareBasicAndDilutedTableTextBlock"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EarningsPerShareAbstract" xlink:to="us-gaap_ScheduleOfEarningsPerShareBasicAndDilutedTableTextBlock" order="0" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note14VoyageVesselOperatingExpensesandCommissionsTables">   
    <loc xlink:type="locator" xlink:label="esea_VesselVoyageAndOperatingExpensesAbstract" xlink:href="esea-20151231.xsd#esea_VesselVoyageAndOperatingExpensesAbstract"/>    
    <loc xlink:type="locator" xlink:label="esea_ScheduleOfVoyageVesselOperatingExpensesAndCommissionsTableTextBlock" xlink:href="esea-20151231.xsd#esea_ScheduleOfVoyageVesselOperatingExpensesAndCommissionsTableTextBlock"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_VesselVoyageAndOperatingExpensesAbstract" xlink:to="esea_ScheduleOfVoyageVesselOperatingExpensesAndCommissionsTableTextBlock" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ScheduleOfSegmentReportingInformationBySegmentTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTextBlock"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_VesselVoyageAndOperatingExpensesAbstract" xlink:to="us-gaap_ScheduleOfSegmentReportingInformationBySegmentTextBlock" order="1" xbrldt:closed="true"/>     
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note15FinancialInstrumentsTables">     
    <loc xlink:type="locator" xlink:label="us-gaap_DisclosureTextBlockSupplementAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DisclosureTextBlockSupplementAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTableTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTableTextBlock"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisclosureTextBlockSupplementAbstract" xlink:to="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTableTextBlock" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueAssetsMeasuredOnRecurringAndNonrecurringBasisTableTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringAndNonrecurringBasisTableTextBlock"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisclosureTextBlockSupplementAbstract" xlink:to="us-gaap_FairValueAssetsMeasuredOnRecurringAndNonrecurringBasisTableTextBlock" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueInputsAssetsQuantitativeInformationTableTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueInputsAssetsQuantitativeInformationTableTextBlock"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisclosureTextBlockSupplementAbstract" xlink:to="us-gaap_FairValueInputsAssetsQuantitativeInformationTableTextBlock" order="2" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note16DerivativeFinancialInstrumentsTables">     
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosureAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosureAbstract"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ScheduleOfDerivativeInstrumentsTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScheduleOfDerivativeInstrumentsTextBlock"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosureAbstract" xlink:to="us-gaap_ScheduleOfDerivativeInstrumentsTextBlock" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ScheduleOfDerivativeInstrumentsInStatementOfFinancialPositionFairValueTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScheduleOfDerivativeInstrumentsInStatementOfFinancialPositionFairValueTextBlock"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosureAbstract" xlink:to="us-gaap_ScheduleOfDerivativeInstrumentsInStatementOfFinancialPositionFairValueTextBlock" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_ScheduleOfDerivativeInstrumentsGainLossInStatementOfFinancialPerformanceTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScheduleOfDerivativeInstrumentsGainLossInStatementOfFinancialPerformanceTextBlock"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosureAbstract" xlink:to="us-gaap_ScheduleOfDerivativeInstrumentsGainLossInStatementOfFinancialPerformanceTextBlock" order="2" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note17InvestmentinJointVentureandOtherInvestmentTables">  
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestmentsAndJointVenturesAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestmentsAndJointVenturesAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestmentsTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestmentsTextBlock"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityMethodInvestmentsAndJointVenturesAbstract" xlink:to="us-gaap_EquityMethodInvestmentsTextBlock" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_InvestmentIncomeTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InvestmentIncomeTextBlock"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityMethodInvestmentsAndJointVenturesAbstract" xlink:to="us-gaap_InvestmentIncomeTextBlock" order="1" xbrldt:closed="true"/>     
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note18PreferredsharesTables">     
    <loc xlink:type="locator" xlink:label="us-gaap_DisclosureTextBlockSupplementAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DisclosureTextBlockSupplementAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ScheduleOfStockholdersEquityTableTextBlock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScheduleOfStockholdersEquityTableTextBlock"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisclosureTextBlockSupplementAbstract" xlink:to="us-gaap_ScheduleOfStockholdersEquityTableTextBlock" order="0" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/CharterersIndividuallyAccountedforMorethan10oftheCompanysVoyageandTimeCharterRevenuesTable">   
    <loc xlink:type="locator" xlink:label="us-gaap_ConcentrationRiskLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ConcentrationRiskLineItems"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_ConcentrationRiskTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ConcentrationRiskTable"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ConcentrationRiskLineItems" xlink:to="us-gaap_ConcentrationRiskTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_MajorCustomersAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_MajorCustomersAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_ConcentrationRiskTable" xlink:to="us-gaap_MajorCustomersAxis" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_NameOfMajorCustomerDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NameOfMajorCustomerDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_MajorCustomersAxis" xlink:to="us-gaap_NameOfMajorCustomerDomain" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_NameOfMajorCustomerDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NameOfMajorCustomerDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_MajorCustomersAxis" xlink:to="us-gaap_NameOfMajorCustomerDomain_0" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ConcentrationRiskByBenchmarkAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_ConcentrationRiskTable" xlink:to="us-gaap_ConcentrationRiskByBenchmarkAxis" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ConcentrationRiskBenchmarkDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_ConcentrationRiskByBenchmarkAxis" xlink:to="us-gaap_ConcentrationRiskBenchmarkDomain" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ConcentrationRiskBenchmarkDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_ConcentrationRiskByBenchmarkAxis" xlink:to="us-gaap_ConcentrationRiskBenchmarkDomain_0" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ConcentrationRiskByTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ConcentrationRiskByTypeAxis"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_ConcentrationRiskTable" xlink:to="us-gaap_ConcentrationRiskByTypeAxis" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ConcentrationRiskTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ConcentrationRiskTypeDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_ConcentrationRiskByTypeAxis" xlink:to="us-gaap_ConcentrationRiskTypeDomain" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ConcentrationRiskTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ConcentrationRiskTypeDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_ConcentrationRiskByTypeAxis" xlink:to="us-gaap_ConcentrationRiskTypeDomain_0" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_SalesRevenueNetMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SalesRevenueNetMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ConcentrationRiskBenchmarkDomain" xlink:to="us-gaap_SalesRevenueNetMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_CustomerConcentrationRiskMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CustomerConcentrationRiskMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ConcentrationRiskTypeDomain" xlink:to="us-gaap_CustomerConcentrationRiskMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_ChartererCMAMember" xlink:href="esea-20151231.xsd#esea_ChartererCMAMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NameOfMajorCustomerDomain" xlink:to="esea_ChartererCMAMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_ChartererGSSMember" xlink:href="esea-20151231.xsd#esea_ChartererGSSMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NameOfMajorCustomerDomain" xlink:to="esea_ChartererGSSMember" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_ChartererMSCMember" xlink:href="esea-20151231.xsd#esea_ChartererMSCMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NameOfMajorCustomerDomain" xlink:to="esea_ChartererMSCMember" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ConcentrationRiskPercentage1" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ConcentrationRiskPercentage1"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ConcentrationRiskLineItems" xlink:to="us-gaap_ConcentrationRiskPercentage1" order="1" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/SummaryofInventoriesTable">   
    <loc xlink:type="locator" xlink:label="us-gaap_InventoryLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InventoryLineItems"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_InventoryCurrentTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InventoryCurrentTable"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_InventoryLineItems" xlink:to="us-gaap_InventoryCurrentTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PublicUtilitiesInventoryAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PublicUtilitiesInventoryAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_InventoryCurrentTable" xlink:to="us-gaap_PublicUtilitiesInventoryAxis" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_PublicUtilitiesInventoryTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PublicUtilitiesInventoryTypeDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PublicUtilitiesInventoryAxis" xlink:to="us-gaap_PublicUtilitiesInventoryTypeDomain" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_PublicUtilitiesInventoryTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PublicUtilitiesInventoryTypeDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PublicUtilitiesInventoryAxis" xlink:to="us-gaap_PublicUtilitiesInventoryTypeDomain_0" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_LubricantMember" xlink:href="esea-20151231.xsd#esea_LubricantMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PublicUtilitiesInventoryTypeDomain" xlink:to="esea_LubricantMember" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_VictuallingMember" xlink:href="esea-20151231.xsd#esea_VictuallingMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PublicUtilitiesInventoryTypeDomain" xlink:to="esea_VictuallingMember" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_BunkersMember" xlink:href="esea-20151231.xsd#esea_BunkersMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PublicUtilitiesInventoryTypeDomain" xlink:to="esea_BunkersMember" order="2" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_InventoryNet" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InventoryNet"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InventoryLineItems" xlink:to="us-gaap_InventoryNet" order="1" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/SummaryofVesselsTable">    
    <loc xlink:type="locator" xlink:label="esea_SummaryOfVesselsAbstract" xlink:href="esea-20151231.xsd#esea_SummaryOfVesselsAbstract"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentGross" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfVesselsAbstract" xlink:to="us-gaap_PropertyPlantAndEquipmentGross" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfVesselsAbstract" xlink:to="us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentNet" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfVesselsAbstract" xlink:to="us-gaap_PropertyPlantAndEquipmentNet" order="2" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_AssetImpairmentChargesBeforeAccumulatedDepreciation" xlink:href="esea-20151231.xsd#esea_AssetImpairmentChargesBeforeAccumulatedDepreciation"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfVesselsAbstract" xlink:to="esea_AssetImpairmentChargesBeforeAccumulatedDepreciation" order="3" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_AccumulatedDepreciationDepletionAndAmortizationImpairmentOfPropertyPlantAndEquipment" xlink:href="esea-20151231.xsd#esea_AccumulatedDepreciationDepletionAndAmortizationImpairmentOfPropertyPlantAndEquipment"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfVesselsAbstract" xlink:to="esea_AccumulatedDepreciationDepletionAndAmortizationImpairmentOfPropertyPlantAndEquipment" order="4" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_AssetImpairmentCharges" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AssetImpairmentCharges"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfVesselsAbstract" xlink:to="us-gaap_AssetImpairmentCharges" order="5" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_Depreciation" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_Depreciation"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfVesselsAbstract" xlink:to="us-gaap_Depreciation" order="6" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentDisposals" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentDisposals"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfVesselsAbstract" xlink:to="us-gaap_PropertyPlantAndEquipmentDisposals" order="7" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_AccumulatedDepreciationDepletionAndAmortizationSaleOfPropertyPlantAndEquipment1" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationSaleOfPropertyPlantAndEquipment1"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfVesselsAbstract" xlink:to="us-gaap_AccumulatedDepreciationDepletionAndAmortizationSaleOfPropertyPlantAndEquipment1" order="8" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_PropertyPlantEquipmentDisposalsNetOfAccumulatedDepreciation" xlink:href="esea-20151231.xsd#esea_PropertyPlantEquipmentDisposalsNetOfAccumulatedDepreciation"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfVesselsAbstract" xlink:to="esea_PropertyPlantEquipmentDisposalsNetOfAccumulatedDepreciation" order="9" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTransfersAndChanges" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTransfersAndChanges"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfVesselsAbstract" xlink:to="us-gaap_PropertyPlantAndEquipmentTransfersAndChanges" order="10" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_AccumulatedDepreciationDepletionAndAmortizationReclassificationsFromPropertyPlantAndEquipment1" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationReclassificationsFromPropertyPlantAndEquipment1"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfVesselsAbstract" xlink:to="us-gaap_AccumulatedDepreciationDepletionAndAmortizationReclassificationsFromPropertyPlantAndEquipment1" order="11" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_PropertyPlantAndEquipmentTransfersAndChangesNet" xlink:href="esea-20151231.xsd#esea_PropertyPlantAndEquipmentTransfersAndChangesNet"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfVesselsAbstract" xlink:to="esea_PropertyPlantAndEquipmentTransfersAndChangesNet" order="12" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentAdditions" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentAdditions"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfVesselsAbstract" xlink:to="us-gaap_PropertyPlantAndEquipmentAdditions" order="13" xbrldt:closed="true"/>    
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/SummaryofDeferredChargesTable">  
    <loc xlink:type="locator" xlink:label="esea_SummaryOfDeferredChargesAbstract" xlink:href="esea-20151231.xsd#esea_SummaryOfDeferredChargesAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_DeferredCosts" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DeferredCosts"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfDeferredChargesAbstract" xlink:to="us-gaap_DeferredCosts" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_AmortizationOfDeferredLoanOriginationFeesNet" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AmortizationOfDeferredLoanOriginationFeesNet"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfDeferredChargesAbstract" xlink:to="us-gaap_AmortizationOfDeferredLoanOriginationFeesNet" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_DeferredOfferingCosts" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DeferredOfferingCosts"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfDeferredChargesAbstract" xlink:to="us-gaap_DeferredOfferingCosts" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_LoanProcessingFee" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LoanProcessingFee"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfDeferredChargesAbstract" xlink:to="us-gaap_LoanProcessingFee" order="3" xbrldt:closed="true"/>    
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/SummaryofAccruedExpensesTable">  
    <loc xlink:type="locator" xlink:label="esea_SummaryOfAccruedExpensesAbstract" xlink:href="esea-20151231.xsd#esea_SummaryOfAccruedExpensesAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_AccruedSalariesCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccruedSalariesCurrent"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfAccruedExpensesAbstract" xlink:to="us-gaap_AccruedSalariesCurrent" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_InterestPayableCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InterestPayableCurrent"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfAccruedExpensesAbstract" xlink:to="us-gaap_InterestPayableCurrent" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_AccruedGeneralAndAdministrativeExpensesCurrent" xlink:href="esea-20151231.xsd#esea_AccruedGeneralAndAdministrativeExpensesCurrent"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfAccruedExpensesAbstract" xlink:to="esea_AccruedGeneralAndAdministrativeExpensesCurrent" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_AccruedSalesCommissionCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccruedSalesCommissionCurrent"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfAccruedExpensesAbstract" xlink:to="us-gaap_AccruedSalesCommissionCurrent" order="3" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_OtherAccruedLiabilitiesCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_OtherAccruedLiabilitiesCurrent"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfAccruedExpensesAbstract" xlink:to="us-gaap_OtherAccruedLiabilitiesCurrent" order="4" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_AccruedLiabilitiesCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccruedLiabilitiesCurrent"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfAccruedExpensesAbstract" xlink:to="us-gaap_AccruedLiabilitiesCurrent" order="5" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/SummaryofLongtermDebtTable">    
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentTable"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_DebtInstrumentLineItems" xlink:to="us-gaap_DebtInstrumentTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_DebtInstrumentTable" xlink:to="us-gaap_DebtInstrumentAxis" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentNameDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DebtInstrumentAxis" xlink:to="us-gaap_DebtInstrumentNameDomain" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentNameDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DebtInstrumentAxis" xlink:to="us-gaap_DebtInstrumentNameDomain_0" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_JoannaMaritimeLtdBorrowerMember" xlink:href="esea-20151231.xsd#esea_JoannaMaritimeLtdBorrowerMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_JoannaMaritimeLtdBorrowerMember" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_ManolisShippingLtdBorrowerMember" xlink:href="esea-20151231.xsd#esea_ManolisShippingLtdBorrowerMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_ManolisShippingLtdBorrowerMember" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_SafConcordShippingLtdBorrowerMember" xlink:href="esea-20151231.xsd#esea_SafConcordShippingLtdBorrowerMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_SafConcordShippingLtdBorrowerMember" order="2" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_PantelisShippingCorpBorrowerMember" xlink:href="esea-20151231.xsd#esea_PantelisShippingCorpBorrowerMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_PantelisShippingCorpBorrowerMember" order="3" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_AggelikiShippingLtdBorrowerMember" xlink:href="esea-20151231.xsd#esea_AggelikiShippingLtdBorrowerMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_AggelikiShippingLtdBorrowerMember" order="4" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_NoumeaShippingLtdBorrowerMember" xlink:href="esea-20151231.xsd#esea_NoumeaShippingLtdBorrowerMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_NoumeaShippingLtdBorrowerMember" order="5" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_EiriniShippingLtdMember" xlink:href="esea-20151231.xsd#esea_EiriniShippingLtdMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_EiriniShippingLtdMember" order="6" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_EuroseasLtdMember" xlink:href="esea-20151231.xsd#esea_EuroseasLtdMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_EuroseasLtdMember" order="7" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentCarryingAmount" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentCarryingAmount"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems" xlink:to="us-gaap_DebtInstrumentCarryingAmount" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_LongTermDebtCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LongTermDebtCurrent"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems" xlink:to="us-gaap_LongTermDebtCurrent" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_LongTermDebtNoncurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LongTermDebtNoncurrent"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems" xlink:to="us-gaap_LongTermDebtNoncurrent" order="3" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/SummaryofFutureAnnualLoanRepaymentsforLongtermDebtTable">   
    <loc xlink:type="locator" xlink:label="esea_SummaryOfFutureAnnualLoanRepaymentsForLongTermDebtAbstract" xlink:href="esea-20151231.xsd#esea_SummaryOfFutureAnnualLoanRepaymentsForLongTermDebtAbstract"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfFutureAnnualLoanRepaymentsForLongTermDebtAbstract" xlink:to="us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfFutureAnnualLoanRepaymentsForLongTermDebtAbstract" xlink:to="us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfFutureAnnualLoanRepaymentsForLongTermDebtAbstract" xlink:to="us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree" order="2" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFour" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFour"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfFutureAnnualLoanRepaymentsForLongTermDebtAbstract" xlink:to="us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFour" order="3" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_LongTermDebt" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LongTermDebt"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfFutureAnnualLoanRepaymentsForLongTermDebtAbstract" xlink:to="us-gaap_LongTermDebt" order="4" xbrldt:closed="true"/>     
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/SummaryoftheStatusoftheCompanysNonvestedSharesTable">    
    <loc xlink:type="locator" xlink:label="esea_Note12StockIncentivePlanDetailsSummaryoftheStatusoftheCompanysNonvestedSharesLineItems" xlink:href="esea-20151231.xsd#esea_Note12StockIncentivePlanDetailsSummaryoftheStatusoftheCompanysNonvestedSharesLineItems"/> 
    <loc xlink:type="locator" xlink:label="esea_Note12StockIncentivePlanDetailsSummaryoftheStatusoftheCompanysNonvestedSharesTable" xlink:href="esea-20151231.xsd#esea_Note12StockIncentivePlanDetailsSummaryoftheStatusoftheCompanysNonvestedSharesTable"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note12StockIncentivePlanDetailsSummaryoftheStatusoftheCompanysNonvestedSharesLineItems" xlink:to="esea_Note12StockIncentivePlanDetailsSummaryoftheStatusoftheCompanysNonvestedSharesTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_AwardTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AwardTypeAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note12StockIncentivePlanDetailsSummaryoftheStatusoftheCompanysNonvestedSharesTable" xlink:to="us-gaap_AwardTypeAxis" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_AwardTypeAxis" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_AwardTypeAxis" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_0" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_RestrictedStockMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RestrictedStockMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:to="us-gaap_RestrictedStockMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note12StockIncentivePlanDetailsSummaryoftheStatusoftheCompanysNonvestedSharesLineItems" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note12StockIncentivePlanDetailsSummaryoftheStatusoftheCompanysNonvestedSharesLineItems" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note12StockIncentivePlanDetailsSummaryoftheStatusoftheCompanysNonvestedSharesLineItems" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod" order="3" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note12StockIncentivePlanDetailsSummaryoftheStatusoftheCompanysNonvestedSharesLineItems" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue" order="4" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note12StockIncentivePlanDetailsSummaryoftheStatusoftheCompanysNonvestedSharesLineItems" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod" order="5" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note12StockIncentivePlanDetailsSummaryoftheStatusoftheCompanysNonvestedSharesLineItems" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue" order="6" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/SummaryofBasicandDilutedLossperCommonShareTable">     
    <loc xlink:type="locator" xlink:label="esea_SummaryOfBasicAndDilutedLossPerCommonShareAbstract" xlink:href="esea-20151231.xsd#esea_SummaryOfBasicAndDilutedLossPerCommonShareAbstract"/>    
    <loc xlink:type="locator" xlink:label="esea_IncomeAbstract" xlink:href="esea-20151231.xsd#esea_IncomeAbstract"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfBasicAndDilutedLossPerCommonShareAbstract" xlink:to="esea_IncomeAbstract" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_IncomeAbstract" xlink:to="us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_BasicEarningsPerShareAbstract" xlink:href="esea-20151231.xsd#esea_BasicEarningsPerShareAbstract"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfBasicAndDilutedLossPerCommonShareAbstract" xlink:to="esea_BasicEarningsPerShareAbstract" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_SummaryOfBasicAndDilutedLossPerCommonShareAbstract" xlink:to="us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding" order="2" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_EarningsPerShareBasic" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EarningsPerShareBasic"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding" xlink:to="us-gaap_EarningsPerShareBasic" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_EffectOfDilutiveSecuritiesAbstract" xlink:href="esea-20151231.xsd#esea_EffectOfDilutiveSecuritiesAbstract"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding" xlink:to="esea_EffectOfDilutiveSecuritiesAbstract" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_EarningsPerShareDiluted" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EarningsPerShareDiluted"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding" xlink:to="us-gaap_EarningsPerShareDiluted" order="2" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/SummaryofVoyageVesselOperatingExpensesandCommissionsTable">     
    <loc xlink:type="locator" xlink:label="esea_Note14VoyageVesselOperatingExpensesandCommissionsDetailsSummaryofVoyageVesselOperatingExpensesandCommissionsLineItems" xlink:href="esea-20151231.xsd#esea_Note14VoyageVesselOperatingExpensesandCommissionsDetailsSummaryofVoyageVesselOperatingExpensesandCommissionsLineItems"/>   
    <loc xlink:type="locator" xlink:label="esea_Note14VoyageVesselOperatingExpensesandCommissionsDetailsSummaryofVoyageVesselOperatingExpensesandCommissionsTable" xlink:href="esea-20151231.xsd#esea_Note14VoyageVesselOperatingExpensesandCommissionsDetailsSummaryofVoyageVesselOperatingExpensesandCommissionsTable"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note14VoyageVesselOperatingExpensesandCommissionsDetailsSummaryofVoyageVesselOperatingExpensesandCommissionsLineItems" xlink:to="esea_Note14VoyageVesselOperatingExpensesandCommissionsDetailsSummaryofVoyageVesselOperatingExpensesandCommissionsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_NatureOfExpenseAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NatureOfExpenseAxis"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note14VoyageVesselOperatingExpensesandCommissionsDetailsSummaryofVoyageVesselOperatingExpensesandCommissionsTable" xlink:to="us-gaap_NatureOfExpenseAxis" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_InterimPeriodCostsNotAllocableDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InterimPeriodCostsNotAllocableDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_NatureOfExpenseAxis" xlink:to="us-gaap_InterimPeriodCostsNotAllocableDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_InterimPeriodCostsNotAllocableDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InterimPeriodCostsNotAllocableDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_NatureOfExpenseAxis" xlink:to="us-gaap_InterimPeriodCostsNotAllocableDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_PortChargesAndCanalDuesMember" xlink:href="esea-20151231.xsd#esea_PortChargesAndCanalDuesMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InterimPeriodCostsNotAllocableDomain" xlink:to="esea_PortChargesAndCanalDuesMember" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_BunkersMember" xlink:href="esea-20151231.xsd#esea_BunkersMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InterimPeriodCostsNotAllocableDomain" xlink:to="esea_BunkersMember" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_CrewWagesAndRelatedCostsMember" xlink:href="esea-20151231.xsd#esea_CrewWagesAndRelatedCostsMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InterimPeriodCostsNotAllocableDomain" xlink:to="esea_CrewWagesAndRelatedCostsMember" order="2" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_InsuranceMember" xlink:href="esea-20151231.xsd#esea_InsuranceMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InterimPeriodCostsNotAllocableDomain" xlink:to="esea_InsuranceMember" order="3" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_RepairsAndMaintenanceMember" xlink:href="esea-20151231.xsd#esea_RepairsAndMaintenanceMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InterimPeriodCostsNotAllocableDomain" xlink:to="esea_RepairsAndMaintenanceMember" order="4" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_LubricantsMember" xlink:href="esea-20151231.xsd#esea_LubricantsMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InterimPeriodCostsNotAllocableDomain" xlink:to="esea_LubricantsMember" order="5" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_SparesAndConsumableStoresMember" xlink:href="esea-20151231.xsd#esea_SparesAndConsumableStoresMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InterimPeriodCostsNotAllocableDomain" xlink:to="esea_SparesAndConsumableStoresMember" order="6" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_ProfessionalAndLegalFeesMember" xlink:href="esea-20151231.xsd#esea_ProfessionalAndLegalFeesMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InterimPeriodCostsNotAllocableDomain" xlink:to="esea_ProfessionalAndLegalFeesMember" order="7" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_OtherVesselOperatingExpensesMember" xlink:href="esea-20151231.xsd#esea_OtherVesselOperatingExpensesMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InterimPeriodCostsNotAllocableDomain" xlink:to="esea_OtherVesselOperatingExpensesMember" order="8" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_VoyageExpensesAbstract" xlink:href="esea-20151231.xsd#esea_VoyageExpensesAbstract"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note14VoyageVesselOperatingExpensesandCommissionsDetailsSummaryofVoyageVesselOperatingExpensesandCommissionsLineItems" xlink:to="esea_VoyageExpensesAbstract" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_VoyageExpenses" xlink:href="esea-20151231.xsd#esea_VoyageExpenses"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_VoyageExpensesAbstract" xlink:to="esea_VoyageExpenses" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_VesselOperatingExpensesAbstract" xlink:href="esea-20151231.xsd#esea_VesselOperatingExpensesAbstract"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note14VoyageVesselOperatingExpensesandCommissionsDetailsSummaryofVoyageVesselOperatingExpensesandCommissionsLineItems" xlink:to="esea_VesselOperatingExpensesAbstract" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_OperatingCostsAndExpenses" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_OperatingCostsAndExpenses"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_VesselOperatingExpensesAbstract" xlink:to="us-gaap_OperatingCostsAndExpenses" order="0" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/CommissionConsistedofCommissionsChargedTable">  
    <loc xlink:type="locator" xlink:label="us-gaap_SegmentReportingInformationLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SegmentReportingInformationLineItems"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_SegmentReportingInformationLineItems" xlink:to="us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_CounterpartyNameAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CounterpartyNameAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable" xlink:to="us-gaap_CounterpartyNameAxis" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_RepurchaseAgreementCounterpartyNameDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RepurchaseAgreementCounterpartyNameDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_CounterpartyNameAxis" xlink:to="us-gaap_RepurchaseAgreementCounterpartyNameDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_RepurchaseAgreementCounterpartyNameDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RepurchaseAgreementCounterpartyNameDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_CounterpartyNameAxis" xlink:to="us-gaap_RepurchaseAgreementCounterpartyNameDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_ThirdPartyMember" xlink:href="esea-20151231.xsd#esea_ThirdPartyMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RepurchaseAgreementCounterpartyNameDomain" xlink:to="esea_ThirdPartyMember" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_RelatedPartyMember" xlink:href="esea-20151231.xsd#esea_RelatedPartyMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RepurchaseAgreementCounterpartyNameDomain" xlink:to="esea_RelatedPartyMember" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PaymentsForCommissions" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PaymentsForCommissions"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SegmentReportingInformationLineItems" xlink:to="us-gaap_PaymentsForCommissions" order="1" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/FairValueofCompanysLiabilitiesTable">   
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems" xlink:to="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueByFairValueHierarchyLevelAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable" xlink:to="us-gaap_FairValueByFairValueHierarchyLevelAxis" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueMeasurementsFairValueHierarchyDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_FairValueByFairValueHierarchyLevelAxis" xlink:to="us-gaap_FairValueMeasurementsFairValueHierarchyDomain" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_FairValueByFairValueHierarchyLevelAxis" xlink:to="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_0" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInstrumentRiskAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable" xlink:to="us-gaap_DerivativeInstrumentRiskAxis" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeContractTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DerivativeInstrumentRiskAxis" xlink:to="us-gaap_DerivativeContractTypeDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeContractTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DerivativeInstrumentRiskAxis" xlink:to="us-gaap_DerivativeContractTypeDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueInputsLevel1Member" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueInputsLevel1Member"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueMeasurementsFairValueHierarchyDomain" xlink:to="us-gaap_FairValueInputsLevel1Member" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueInputsLevel2Member" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueInputsLevel2Member"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueMeasurementsFairValueHierarchyDomain" xlink:to="us-gaap_FairValueInputsLevel2Member" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueInputsLevel3Member" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueInputsLevel3Member"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueMeasurementsFairValueHierarchyDomain" xlink:to="us-gaap_FairValueInputsLevel3Member" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_InterestRateSwapMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InterestRateSwapMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain" xlink:to="us-gaap_InterestRateSwapMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_LiabilitiesAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LiabilitiesAbstract"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems" xlink:to="us-gaap_LiabilitiesAbstract" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeFairValueOfDerivativeLiability" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeFairValueOfDerivativeLiability"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems" xlink:to="us-gaap_DerivativeFairValueOfDerivativeLiability" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_LiabilitiesAbstract0" xlink:href="esea-20151231.xsd#esea_LiabilitiesAbstract0"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems" xlink:to="esea_LiabilitiesAbstract0" order="3" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/AssetMeasuredatFairValueonaNonrecurringBasisTable">  
    <loc xlink:type="locator" xlink:label="esea_Note15FinancialInstrumentsDetailsAssetMeasuredatFairValueonaNonrecurringBasisLineItems" xlink:href="esea-20151231.xsd#esea_Note15FinancialInstrumentsDetailsAssetMeasuredatFairValueonaNonrecurringBasisLineItems"/> 
    <loc xlink:type="locator" xlink:label="esea_Note15FinancialInstrumentsDetailsAssetMeasuredatFairValueonaNonrecurringBasisTable" xlink:href="esea-20151231.xsd#esea_Note15FinancialInstrumentsDetailsAssetMeasuredatFairValueonaNonrecurringBasisTable"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note15FinancialInstrumentsDetailsAssetMeasuredatFairValueonaNonrecurringBasisLineItems" xlink:to="esea_Note15FinancialInstrumentsDetailsAssetMeasuredatFairValueonaNonrecurringBasisTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueByFairValueHierarchyLevelAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note15FinancialInstrumentsDetailsAssetMeasuredatFairValueonaNonrecurringBasisTable" xlink:to="us-gaap_FairValueByFairValueHierarchyLevelAxis" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueMeasurementsFairValueHierarchyDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_FairValueByFairValueHierarchyLevelAxis" xlink:to="us-gaap_FairValueMeasurementsFairValueHierarchyDomain" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_FairValueByFairValueHierarchyLevelAxis" xlink:to="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_0" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note15FinancialInstrumentsDetailsAssetMeasuredatFairValueonaNonrecurringBasisTable" xlink:to="us-gaap_PropertyPlantAndEquipmentByTypeAxis" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueInputsLevel2Member" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueInputsLevel2Member"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueMeasurementsFairValueHierarchyDomain" xlink:to="us-gaap_FairValueInputsLevel2Member" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_MVAristidesMember" xlink:href="esea-20151231.xsd#esea_MVAristidesMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_MVAristidesMember" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentFairValueDisclosure" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentFairValueDisclosure"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note15FinancialInstrumentsDetailsAssetMeasuredatFairValueonaNonrecurringBasisLineItems" xlink:to="us-gaap_PropertyPlantAndEquipmentFairValueDisclosure" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_AssetImpairmentCharges" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AssetImpairmentCharges"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note15FinancialInstrumentsDetailsAssetMeasuredatFairValueonaNonrecurringBasisLineItems" xlink:to="us-gaap_AssetImpairmentCharges" order="2" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/QuantitativeInformationaboutLevel3FairValueMeasurementsTable">   
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueInputsAssetsQuantitativeInformationLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueInputsAssetsQuantitativeInformationLineItems"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueInputsAssetsQuantitativeInformationTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueInputsAssetsQuantitativeInformationTable"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_FairValueInputsAssetsQuantitativeInformationLineItems" xlink:to="us-gaap_FairValueInputsAssetsQuantitativeInformationTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueByFairValueHierarchyLevelAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_FairValueInputsAssetsQuantitativeInformationTable" xlink:to="us-gaap_FairValueByFairValueHierarchyLevelAxis" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueMeasurementsFairValueHierarchyDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_FairValueByFairValueHierarchyLevelAxis" xlink:to="us-gaap_FairValueMeasurementsFairValueHierarchyDomain" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_FairValueByFairValueHierarchyLevelAxis" xlink:to="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_0" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ValuationTechniqueAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ValuationTechniqueAxis"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_FairValueInputsAssetsQuantitativeInformationTable" xlink:to="us-gaap_ValuationTechniqueAxis" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ValuationTechniqueDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ValuationTechniqueDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_ValuationTechniqueAxis" xlink:to="us-gaap_ValuationTechniqueDomain" order="0" xbrldt:closed="true"/>  
<!-- [WMV4][XcpuxYn59dz7vgYynUDuwG7r97K2jUTysl7vcz0lJt8UCLPdYZl35nXbOCDIyGTq0KnTsIHf38wU0uD4ZRE85Ekj/WyiGtWybpv1FN0Ijp9gwXy4RJI14a/MzIVYUrwhkN23EBmnwC+BciCHUvJ/5T7boSmwUaFkqLLHLNuoe2B55GYertuublM1C+voV1wHy9Oani92ZAaWqS605vhgL1NMhUCnHZ+cqG5IRf2ybokmkC2OxzVPVg==] CSR-->    
    <loc xlink:type="locator" xlink:label="us-gaap_ValuationTechniqueDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ValuationTechniqueDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_ValuationTechniqueAxis" xlink:to="us-gaap_ValuationTechniqueDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueInputsLevel3Member" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueInputsLevel3Member"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueMeasurementsFairValueHierarchyDomain" xlink:to="us-gaap_FairValueInputsLevel3Member" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_DiscountedCashFlowMember" xlink:href="esea-20151231.xsd#esea_DiscountedCashFlowMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ValuationTechniqueDomain" xlink:to="esea_DiscountedCashFlowMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_InvestmentsAndOtherNoncurrentAssets" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InvestmentsAndOtherNoncurrentAssets"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueInputsAssetsQuantitativeInformationLineItems" xlink:to="us-gaap_InvestmentsAndOtherNoncurrentAssets" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueMeasurementsValuationTechniques" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueMeasurementsValuationTechniques"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueInputsAssetsQuantitativeInformationLineItems" xlink:to="us-gaap_FairValueMeasurementsValuationTechniques" order="2" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/SummaryofDerivativeActivityTable">     
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeLineItems"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeTable"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_DerivativeLineItems" xlink:to="us-gaap_DerivativeTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInstrumentRiskAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_DerivativeTable" xlink:to="us-gaap_DerivativeInstrumentRiskAxis" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeContractTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DerivativeInstrumentRiskAxis" xlink:to="us-gaap_DerivativeContractTypeDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeContractTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DerivativeInstrumentRiskAxis" xlink:to="us-gaap_DerivativeContractTypeDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_CounterpartyNameAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CounterpartyNameAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_DerivativeTable" xlink:to="us-gaap_CounterpartyNameAxis" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_RepurchaseAgreementCounterpartyNameDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RepurchaseAgreementCounterpartyNameDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_CounterpartyNameAxis" xlink:to="us-gaap_RepurchaseAgreementCounterpartyNameDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_RepurchaseAgreementCounterpartyNameDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RepurchaseAgreementCounterpartyNameDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_CounterpartyNameAxis" xlink:to="us-gaap_RepurchaseAgreementCounterpartyNameDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_InterestRateSwapOneMember" xlink:href="esea-20151231.xsd#esea_InterestRateSwapOneMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain" xlink:to="esea_InterestRateSwapOneMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_InterestRateSwapTwoMember" xlink:href="esea-20151231.xsd#esea_InterestRateSwapTwoMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain" xlink:to="esea_InterestRateSwapTwoMember" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_InterestRateSwapThreeMember" xlink:href="esea-20151231.xsd#esea_InterestRateSwapThreeMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain" xlink:to="esea_InterestRateSwapThreeMember" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_EurobankMember" xlink:href="esea-20151231.xsd#esea_EurobankMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RepurchaseAgreementCounterpartyNameDomain" xlink:to="esea_EurobankMember" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInceptionDates" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInceptionDates"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeLineItems" xlink:to="us-gaap_DerivativeInceptionDates" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="invest_DerivativeNotionalAmount" xlink:href="http://xbrl.sec.gov/invest/2013/invest-2013-01-31.xsd#invest_DerivativeNotionalAmount"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeLineItems" xlink:to="invest_DerivativeNotionalAmount" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeFixedInterestRate" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeFixedInterestRate"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeLineItems" xlink:to="us-gaap_DerivativeFixedInterestRate" order="3" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeMaturityDates" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeMaturityDates"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeLineItems" xlink:to="us-gaap_DerivativeMaturityDates" order="4" xbrldt:closed="true"/>     
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/DerivativeNotDesignatedasHedgingInstrumentsbyAccountTypeTable">    
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativesFairValueLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativesFairValueLineItems"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_DerivativesFairValueLineItems" xlink:to="us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInstrumentRiskAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable" xlink:to="us-gaap_DerivativeInstrumentRiskAxis" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeContractTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DerivativeInstrumentRiskAxis" xlink:to="us-gaap_DerivativeContractTypeDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeContractTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DerivativeInstrumentRiskAxis" xlink:to="us-gaap_DerivativeContractTypeDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_InterestRateSwapMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InterestRateSwapMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain" xlink:to="us-gaap_InterestRateSwapMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeLiabilitiesCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeLiabilitiesCurrent"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativesFairValueLineItems" xlink:to="us-gaap_DerivativeLiabilitiesCurrent" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeLiabilitiesNoncurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeLiabilitiesNoncurrent"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativesFairValueLineItems" xlink:to="us-gaap_DerivativeLiabilitiesNoncurrent" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeLiabilities" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeLiabilities"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativesFairValueLineItems" xlink:to="us-gaap_DerivativeLiabilities" order="3" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/GainorLossonDerivativesNotDesignatedasHedgingInstrumentsTable">    
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInstrumentsGainLossLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInstrumentsGainLossLineItems"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipByIncomeStatementLocationByDerivativeInstrumentRiskTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipByIncomeStatementLocationByDerivativeInstrumentRiskTable"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_DerivativeInstrumentsGainLossLineItems" xlink:to="us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipByIncomeStatementLocationByDerivativeInstrumentRiskTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_HedgingDesignationAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_HedgingDesignationAxis"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipByIncomeStatementLocationByDerivativeInstrumentRiskTable" xlink:to="us-gaap_HedgingDesignationAxis" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_HedgingDesignationDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_HedgingDesignationDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_HedgingDesignationAxis" xlink:to="us-gaap_HedgingDesignationDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_HedgingDesignationDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_HedgingDesignationDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_HedgingDesignationAxis" xlink:to="us-gaap_HedgingDesignationDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInstrumentRiskAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipByIncomeStatementLocationByDerivativeInstrumentRiskTable" xlink:to="us-gaap_DerivativeInstrumentRiskAxis" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeContractTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DerivativeInstrumentRiskAxis" xlink:to="us-gaap_DerivativeContractTypeDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeContractTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DerivativeInstrumentRiskAxis" xlink:to="us-gaap_DerivativeContractTypeDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_InterestRateContractMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InterestRateContractMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain" xlink:to="us-gaap_InterestRateContractMember" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_NondesignatedMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NondesignatedMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_HedgingDesignationDomain" xlink:to="us-gaap_NondesignatedMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInstrumentsNotDesignatedAsHedgingInstrumentsGain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInstrumentsNotDesignatedAsHedgingInstrumentsGain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeInstrumentsGainLossLineItems" xlink:to="us-gaap_DerivativeInstrumentsNotDesignatedAsHedgingInstrumentsGain" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInstrumentsNotDesignatedAsHedgingInstrumentsLoss" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInstrumentsNotDesignatedAsHedgingInstrumentsLoss"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeInstrumentsGainLossLineItems" xlink:to="us-gaap_DerivativeInstrumentsNotDesignatedAsHedgingInstrumentsLoss" order="2" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInstrumentsNotDesignatedAsHedgingInstrumentsGainLossNet" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInstrumentsNotDesignatedAsHedgingInstrumentsGainLossNet"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeInstrumentsGainLossLineItems" xlink:to="us-gaap_DerivativeInstrumentsNotDesignatedAsHedgingInstrumentsGainLossNet" order="3" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/SummarizedFinancialInformationfortheJointVentureTable"> 
    <loc xlink:type="locator" xlink:label="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScheduleOfEquityMethodInvestmentsLineItems"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ScheduleOfEquityMethodInvestmentsTable" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScheduleOfEquityMethodInvestmentsTable"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems" xlink:to="us-gaap_ScheduleOfEquityMethodInvestmentsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsTable" xlink:to="us-gaap_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvesteeNameDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvesteeNameDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis" xlink:to="us-gaap_EquityMethodInvesteeNameDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvesteeNameDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvesteeNameDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis" xlink:to="us-gaap_EquityMethodInvesteeNameDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_EuromarLLCTheJointVentureMember" xlink:href="esea-20151231.xsd#esea_EuromarLLCTheJointVentureMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityMethodInvesteeNameDomain" xlink:to="esea_EuromarLLCTheJointVentureMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationCurrentAssets" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestmentSummarizedFinancialInformationCurrentAssets"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems" xlink:to="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationCurrentAssets" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationNoncurrentAssets" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestmentSummarizedFinancialInformationNoncurrentAssets"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems" xlink:to="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationNoncurrentAssets" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationCurrentLiabilities" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestmentSummarizedFinancialInformationCurrentLiabilities"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems" xlink:to="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationCurrentLiabilities" order="3" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationNoncurrentLiabilities" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestmentSummarizedFinancialInformationNoncurrentLiabilities"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems" xlink:to="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationNoncurrentLiabilities" order="4" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationEquity" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestmentSummarizedFinancialInformationEquity"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems" xlink:to="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationEquity" order="5" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_VoyageRevenues" xlink:href="esea-20151231.xsd#esea_VoyageRevenues"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems" xlink:to="esea_VoyageRevenues" order="6" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationRevenue" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestmentSummarizedFinancialInformationRevenue"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems" xlink:to="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationRevenue" order="7" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationIncomeLossFromContinuingOperationsBeforeExtraordinaryItems" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestmentSummarizedFinancialInformationIncomeLossFromContinuingOperationsBeforeExtraordinaryItems"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems" xlink:to="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationIncomeLossFromContinuingOperationsBeforeExtraordinaryItems" order="8" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationNetIncomeLoss" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestmentSummarizedFinancialInformationNetIncomeLoss"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems" xlink:to="us-gaap_EquityMethodInvestmentSummarizedFinancialInformationNetIncomeLoss" order="9" xbrldt:closed="true"/>     
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/InvestmentinJointVentureTable">  
    <loc xlink:type="locator" xlink:label="esea_InvestmentInJointVentureAbstract" xlink:href="esea-20151231.xsd#esea_InvestmentInJointVentureAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_InvestmentsAndOtherNoncurrentAssets" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InvestmentsAndOtherNoncurrentAssets"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_InvestmentInJointVentureAbstract" xlink:to="us-gaap_InvestmentsAndOtherNoncurrentAssets" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_InvestmentIncomeNet" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InvestmentIncomeNet"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_InvestmentInJointVentureAbstract" xlink:to="us-gaap_InvestmentIncomeNet" order="1" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/DividendsSeriesBPreferredSharesTable">    
    <loc xlink:type="locator" xlink:label="esea_Note18PreferredsharesDetailsDividendsSeriesBPreferredSharesLineItems" xlink:href="esea-20151231.xsd#esea_Note18PreferredsharesDetailsDividendsSeriesBPreferredSharesLineItems"/>     
    <loc xlink:type="locator" xlink:label="esea_Note18PreferredsharesDetailsDividendsSeriesBPreferredSharesTable" xlink:href="esea-20151231.xsd#esea_Note18PreferredsharesDetailsDividendsSeriesBPreferredSharesTable"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note18PreferredsharesDetailsDividendsSeriesBPreferredSharesLineItems" xlink:to="esea_Note18PreferredsharesDetailsDividendsSeriesBPreferredSharesTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_StatementClassOfStockAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note18PreferredsharesDetailsDividendsSeriesBPreferredSharesTable" xlink:to="us-gaap_StatementClassOfStockAxis" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ClassOfStockDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ClassOfStockDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementClassOfStockAxis" xlink:to="us-gaap_ClassOfStockDomain" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ClassOfStockDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ClassOfStockDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementClassOfStockAxis" xlink:to="us-gaap_ClassOfStockDomain_0" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_SubsidiarySaleOfStockAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note18PreferredsharesDetailsDividendsSeriesBPreferredSharesTable" xlink:to="us-gaap_SubsidiarySaleOfStockAxis" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_SaleOfStockNameOfTransactionDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_SubsidiarySaleOfStockAxis" xlink:to="us-gaap_SaleOfStockNameOfTransactionDomain" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_SaleOfStockNameOfTransactionDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_SubsidiarySaleOfStockAxis" xlink:to="us-gaap_SaleOfStockNameOfTransactionDomain_0" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_StatementEquityComponentsAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note18PreferredsharesDetailsDividendsSeriesBPreferredSharesTable" xlink:to="us-gaap_StatementEquityComponentsAxis" order="2" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_EquityComponentDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityComponentDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementEquityComponentsAxis" xlink:to="us-gaap_EquityComponentDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_EquityComponentDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityComponentDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementEquityComponentsAxis" xlink:to="us-gaap_EquityComponentDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PreferredStockMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain" xlink:to="us-gaap_PreferredStockMember" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PrivatePlacementMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PrivatePlacementMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SaleOfStockNameOfTransactionDomain" xlink:to="us-gaap_PrivatePlacementMember" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_OrdinaryPreferredStockMember" xlink:href="esea-20151231.xsd#esea_OrdinaryPreferredStockMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain" xlink:to="esea_OrdinaryPreferredStockMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_PreferredStockIssuedAsDividendsMember" xlink:href="esea-20151231.xsd#esea_PreferredStockIssuedAsDividendsMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain" xlink:to="esea_PreferredStockIssuedAsDividendsMember" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_StockIssuedDuringPeriodSharesNewIssues" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsDividendsSeriesBPreferredSharesLineItems" xlink:to="us-gaap_StockIssuedDuringPeriodSharesNewIssues" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_StockIssuedDuringPeriodValueNewIssues" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueNewIssues"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsDividendsSeriesBPreferredSharesLineItems" xlink:to="us-gaap_StockIssuedDuringPeriodValueNewIssues" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_PreferredStockDividendsShares" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockDividendsShares"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsDividendsSeriesBPreferredSharesLineItems" xlink:to="us-gaap_PreferredStockDividendsShares" order="3" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DividendsPreferredStockPaidinkind" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DividendsPreferredStockPaidinkind"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsDividendsSeriesBPreferredSharesLineItems" xlink:to="us-gaap_DividendsPreferredStockPaidinkind" order="4" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PreferredStockSharesOutstanding" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockSharesOutstanding"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsDividendsSeriesBPreferredSharesLineItems" xlink:to="us-gaap_PreferredStockSharesOutstanding" order="5" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PreferredStockValue" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockValue"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsDividendsSeriesBPreferredSharesLineItems" xlink:to="us-gaap_PreferredStockValue" order="6" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note1BasisofPresentationandGeneralInformationDetails">     
    <loc xlink:type="locator" xlink:label="esea_Note1BasisofPresentationandGeneralInformationDetailsLineItems" xlink:href="esea-20151231.xsd#esea_Note1BasisofPresentationandGeneralInformationDetailsLineItems"/> 
    <loc xlink:type="locator" xlink:label="esea_Note1BasisofPresentationandGeneralInformationDetailsTable" xlink:href="esea-20151231.xsd#esea_Note1BasisofPresentationandGeneralInformationDetailsTable"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note1BasisofPresentationandGeneralInformationDetailsLineItems" xlink:to="esea_Note1BasisofPresentationandGeneralInformationDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_NonmonetaryTransactionTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NonmonetaryTransactionTypeAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note1BasisofPresentationandGeneralInformationDetailsTable" xlink:to="us-gaap_NonmonetaryTransactionTypeAxis" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_NonmonetaryTransactionTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NonmonetaryTransactionTypeDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_NonmonetaryTransactionTypeAxis" xlink:to="us-gaap_NonmonetaryTransactionTypeDomain" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_NonmonetaryTransactionTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NonmonetaryTransactionTypeDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_NonmonetaryTransactionTypeAxis" xlink:to="us-gaap_NonmonetaryTransactionTypeDomain_0" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="dei_LegalEntityAxis" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_LegalEntityAxis"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note1BasisofPresentationandGeneralInformationDetailsTable" xlink:to="dei_LegalEntityAxis" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="dei_EntityDomain" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="dei_LegalEntityAxis" xlink:to="dei_EntityDomain" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="dei_EntityDomain_0" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="dei_LegalEntityAxis" xlink:to="dei_EntityDomain_0" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_ReverseStockSplitMember" xlink:href="esea-20151231.xsd#esea_ReverseStockSplitMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NonmonetaryTransactionTypeDomain" xlink:to="esea_ReverseStockSplitMember" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_FriendsInvestmentCompanyIncMember" xlink:href="esea-20151231.xsd#esea_FriendsInvestmentCompanyIncMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_EntityDomain" xlink:to="esea_FriendsInvestmentCompanyIncMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_MinorityInterestOwnershipPercentageByNoncontrollingOwners" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_MinorityInterestOwnershipPercentageByNoncontrollingOwners"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note1BasisofPresentationandGeneralInformationDetailsLineItems" xlink:to="us-gaap_MinorityInterestOwnershipPercentageByNoncontrollingOwners" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_StockholdersEquityNoteStockSplitConversionRatio1" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockholdersEquityNoteStockSplitConversionRatio1"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note1BasisofPresentationandGeneralInformationDetailsLineItems" xlink:to="us-gaap_StockholdersEquityNoteStockSplitConversionRatio1" order="2" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_NewSharesDueToRoundingReverseStockSplit" xlink:href="esea-20151231.xsd#esea_NewSharesDueToRoundingReverseStockSplit"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note1BasisofPresentationandGeneralInformationDetailsLineItems" xlink:to="esea_NewSharesDueToRoundingReverseStockSplit" order="3" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note2SignificantAccountingPoliciesDetails">    
    <loc xlink:type="locator" xlink:label="esea_Note2SignificantAccountingPoliciesDetailsLineItems" xlink:href="esea-20151231.xsd#esea_Note2SignificantAccountingPoliciesDetailsLineItems"/>    
    <loc xlink:type="locator" xlink:label="esea_Note2SignificantAccountingPoliciesDetailsTable" xlink:href="esea-20151231.xsd#esea_Note2SignificantAccountingPoliciesDetailsTable"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note2SignificantAccountingPoliciesDetailsLineItems" xlink:to="esea_Note2SignificantAccountingPoliciesDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_RangeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RangeAxis"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note2SignificantAccountingPoliciesDetailsTable" xlink:to="us-gaap_RangeAxis" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_RangeMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RangeMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_RangeAxis" xlink:to="us-gaap_RangeMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_RangeMember_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RangeMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_RangeAxis" xlink:to="us-gaap_RangeMember_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note2SignificantAccountingPoliciesDetailsTable" xlink:to="us-gaap_PropertyPlantAndEquipmentByTypeAxis" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_MinimumMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_MinimumMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RangeMember" xlink:to="us-gaap_MinimumMember" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_VesselsMember" xlink:href="esea-20151231.xsd#esea_VesselsMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_VesselsMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentUsefulLife" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentUsefulLife"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note2SignificantAccountingPoliciesDetailsLineItems" xlink:to="us-gaap_PropertyPlantAndEquipmentUsefulLife" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_ShipOwningCrewContractTerm" xlink:href="esea-20151231.xsd#esea_ShipOwningCrewContractTerm"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note2SignificantAccountingPoliciesDetailsLineItems" xlink:to="esea_ShipOwningCrewContractTerm" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_NumberOfReportableSegments" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NumberOfReportableSegments"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note2SignificantAccountingPoliciesDetailsLineItems" xlink:to="us-gaap_NumberOfReportableSegments" order="3" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note4AdvancesforVesselsunderConstructionDetails">     
    <loc xlink:type="locator" xlink:label="esea_Note4AdvancesforVesselsunderConstructionDetailsLineItems" xlink:href="esea-20151231.xsd#esea_Note4AdvancesforVesselsunderConstructionDetailsLineItems"/> 
    <loc xlink:type="locator" xlink:label="esea_Note4AdvancesforVesselsunderConstructionDetailsTable" xlink:href="esea-20151231.xsd#esea_Note4AdvancesforVesselsunderConstructionDetailsTable"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note4AdvancesforVesselsunderConstructionDetailsLineItems" xlink:to="esea_Note4AdvancesforVesselsunderConstructionDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note4AdvancesforVesselsunderConstructionDetailsTable" xlink:to="us-gaap_PropertyPlantAndEquipmentByTypeAxis" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_UltramaxDrybulkCarriersMember" xlink:href="esea-20151231.xsd#esea_UltramaxDrybulkCarriersMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_UltramaxDrybulkCarriersMember" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_KamsarmaxDrybulkCarriersMember" xlink:href="esea-20151231.xsd#esea_KamsarmaxDrybulkCarriersMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_KamsarmaxDrybulkCarriersMember" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_NumberOfNewBuildingVessels" xlink:href="esea-20151231.xsd#esea_NumberOfNewBuildingVessels"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note4AdvancesforVesselsunderConstructionDetailsLineItems" xlink:to="esea_NumberOfNewBuildingVessels" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_VesselCarryingCapacity" xlink:href="esea-20151231.xsd#esea_VesselCarryingCapacity"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note4AdvancesforVesselsunderConstructionDetailsLineItems" xlink:to="esea_VesselCarryingCapacity" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_LongTermPurchaseCommitmentAmount" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LongTermPurchaseCommitmentAmount"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note4AdvancesforVesselsunderConstructionDetailsLineItems" xlink:to="us-gaap_LongTermPurchaseCommitmentAmount" order="3" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_AdvancesForVesselAcquisitionNetOfCapitalizedCosts" xlink:href="esea-20151231.xsd#esea_AdvancesForVesselAcquisitionNetOfCapitalizedCosts"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note4AdvancesforVesselsunderConstructionDetailsLineItems" xlink:to="esea_AdvancesForVesselAcquisitionNetOfCapitalizedCosts" order="4" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_AccumulatedCapitalizedInterestCosts" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccumulatedCapitalizedInterestCosts"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note4AdvancesforVesselsunderConstructionDetailsLineItems" xlink:to="us-gaap_AccumulatedCapitalizedInterestCosts" order="5" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_AdvancesForVesselAcquisition" xlink:href="esea-20151231.xsd#esea_AdvancesForVesselAcquisition"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note4AdvancesforVesselsunderConstructionDetailsLineItems" xlink:to="esea_AdvancesForVesselAcquisition" order="6" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note5VesselsNetDetails">     
    <loc xlink:type="locator" xlink:label="esea_Note5VesselsNetDetailsLineItems" xlink:href="esea-20151231.xsd#esea_Note5VesselsNetDetailsLineItems"/> 
    <loc xlink:type="locator" xlink:label="esea_Note5VesselsNetDetailsTable" xlink:href="esea-20151231.xsd#esea_Note5VesselsNetDetailsTable"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note5VesselsNetDetailsLineItems" xlink:to="esea_Note5VesselsNetDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_RangeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RangeAxis"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note5VesselsNetDetailsTable" xlink:to="us-gaap_RangeAxis" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_RangeMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RangeMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_RangeAxis" xlink:to="us-gaap_RangeMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_RangeMember_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RangeMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_RangeAxis" xlink:to="us-gaap_RangeMember_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note5VesselsNetDetailsTable" xlink:to="us-gaap_PropertyPlantAndEquipmentByTypeAxis" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ChangeInAccountingEstimateByTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ChangeInAccountingEstimateByTypeAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note5VesselsNetDetailsTable" xlink:to="us-gaap_ChangeInAccountingEstimateByTypeAxis" order="2" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ChangeInAccountingEstimateTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ChangeInAccountingEstimateTypeDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_ChangeInAccountingEstimateByTypeAxis" xlink:to="us-gaap_ChangeInAccountingEstimateTypeDomain" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ChangeInAccountingEstimateTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ChangeInAccountingEstimateTypeDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_ChangeInAccountingEstimateByTypeAxis" xlink:to="us-gaap_ChangeInAccountingEstimateTypeDomain_0" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_StatementScenarioAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementScenarioAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note5VesselsNetDetailsTable" xlink:to="us-gaap_StatementScenarioAxis" order="3" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_MaximumMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_MaximumMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RangeMember" xlink:to="us-gaap_MaximumMember" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_MinimumMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_MinimumMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RangeMember" xlink:to="us-gaap_MinimumMember" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_PayableToManagerMember" xlink:href="esea-20151231.xsd#esea_PayableToManagerMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScenarioUnspecifiedDomain" xlink:to="esea_PayableToManagerMember" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_MVEiriniPMember" xlink:href="esea-20151231.xsd#esea_MVEiriniPMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_MVEiriniPMember" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_TigerBridgeMember" xlink:href="esea-20151231.xsd#esea_TigerBridgeMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_TigerBridgeMember" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_MarinosMember" xlink:href="esea-20151231.xsd#esea_MarinosMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_MarinosMember" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_DespinaPMember" xlink:href="esea-20151231.xsd#esea_DespinaPMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_DespinaPMember" order="3" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_MVAristidesMember" xlink:href="esea-20151231.xsd#esea_MVAristidesMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_MVAristidesMember" order="4" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_ContainershipsMember" xlink:href="esea-20151231.xsd#esea_ContainershipsMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_ContainershipsMember" order="5" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_MidCycleConditionContainershipsMember" xlink:href="esea-20151231.xsd#esea_MidCycleConditionContainershipsMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_MidCycleConditionContainershipsMember" order="6" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_VesselsMember" xlink:href="esea-20151231.xsd#esea_VesselsMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_VesselsMember" order="7" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ServiceLifeMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ServiceLifeMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ChangeInAccountingEstimateTypeDomain" xlink:to="us-gaap_ServiceLifeMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_NumberOfVesselsAcquired" xlink:href="esea-20151231.xsd#esea_NumberOfVesselsAcquired"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note5VesselsNetDetailsLineItems" xlink:to="esea_NumberOfVesselsAcquired" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PaymentsToAcquirePropertyPlantAndEquipment" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note5VesselsNetDetailsLineItems" xlink:to="us-gaap_PaymentsToAcquirePropertyPlantAndEquipment" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_NumberOfVesselsDisposedOrSold" xlink:href="esea-20151231.xsd#esea_NumberOfVesselsDisposedOrSold"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note5VesselsNetDetailsLineItems" xlink:to="esea_NumberOfVesselsDisposedOrSold" order="3" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note5VesselsNetDetailsLineItems" xlink:to="us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment" order="4" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_SalesCommissionPercentage" xlink:href="esea-20151231.xsd#esea_SalesCommissionPercentage"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note5VesselsNetDetailsLineItems" xlink:to="esea_SalesCommissionPercentage" order="5" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_GainLossOnSaleOfPropertyPlantEquipment" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_GainLossOnSaleOfPropertyPlantEquipment"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note5VesselsNetDetailsLineItems" xlink:to="us-gaap_GainLossOnSaleOfPropertyPlantEquipment" order="6" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_AssetsHeldForSaleNotPartOfDisposalGroupCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AssetsHeldForSaleNotPartOfDisposalGroupCurrent"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note5VesselsNetDetailsLineItems" xlink:to="us-gaap_AssetsHeldForSaleNotPartOfDisposalGroupCurrent" order="7" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_LiabilitiesOfDisposalGroupIncludingDiscontinuedOperationCurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LiabilitiesOfDisposalGroupIncludingDiscontinuedOperationCurrent"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note5VesselsNetDetailsLineItems" xlink:to="us-gaap_LiabilitiesOfDisposalGroupIncludingDiscontinuedOperationCurrent" order="8" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_PropertyPlantAndEquipmentUsefulLifeDisposalGroup" xlink:href="esea-20151231.xsd#esea_PropertyPlantAndEquipmentUsefulLifeDisposalGroup"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note5VesselsNetDetailsLineItems" xlink:to="esea_PropertyPlantAndEquipmentUsefulLifeDisposalGroup" order="9" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentUsefulLife" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentUsefulLife"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note5VesselsNetDetailsLineItems" xlink:to="us-gaap_PropertyPlantAndEquipmentUsefulLife" order="10" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DepreciationDepletionAndAmortization" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DepreciationDepletionAndAmortization"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note5VesselsNetDetailsLineItems" xlink:to="us-gaap_DepreciationDepletionAndAmortization" order="11" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_EarningsPerShareBasicAndDiluted" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EarningsPerShareBasicAndDiluted"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note5VesselsNetDetailsLineItems" xlink:to="us-gaap_EarningsPerShareBasicAndDiluted" order="12" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_AssetImpairmentCharges" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AssetImpairmentCharges"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note5VesselsNetDetailsLineItems" xlink:to="us-gaap_AssetImpairmentCharges" order="13" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_PropertyPlantAndEquipmentPledgedAsCollateral" xlink:href="esea-20151231.xsd#esea_PropertyPlantAndEquipmentPledgedAsCollateral"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note5VesselsNetDetailsLineItems" xlink:to="esea_PropertyPlantAndEquipmentPledgedAsCollateral" order="14" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note8RelatedPartyTransactionsDetails">    
    <loc xlink:type="locator" xlink:label="esea_Note8RelatedPartyTransactionsDetailsLineItems" xlink:href="esea-20151231.xsd#esea_Note8RelatedPartyTransactionsDetailsLineItems"/>    
    <loc xlink:type="locator" xlink:label="esea_Note8RelatedPartyTransactionsDetailsTable" xlink:href="esea-20151231.xsd#esea_Note8RelatedPartyTransactionsDetailsTable"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note8RelatedPartyTransactionsDetailsLineItems" xlink:to="esea_Note8RelatedPartyTransactionsDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="dei_LegalEntityAxis" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_LegalEntityAxis"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note8RelatedPartyTransactionsDetailsTable" xlink:to="dei_LegalEntityAxis" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="dei_EntityDomain" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="dei_LegalEntityAxis" xlink:to="dei_EntityDomain" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="dei_EntityDomain_0" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="dei_LegalEntityAxis" xlink:to="dei_EntityDomain_0" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_RelatedPartyTransactionAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RelatedPartyTransactionAxis"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note8RelatedPartyTransactionsDetailsTable" xlink:to="us-gaap_RelatedPartyTransactionAxis" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_RelatedPartyTransactionDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RelatedPartyTransactionDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_RelatedPartyTransactionAxis" xlink:to="us-gaap_RelatedPartyTransactionDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_RelatedPartyTransactionDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RelatedPartyTransactionDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_RelatedPartyTransactionAxis" xlink:to="us-gaap_RelatedPartyTransactionDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note8RelatedPartyTransactionsDetailsTable" xlink:to="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis" order="2" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_RelatedPartyDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RelatedPartyDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis" xlink:to="us-gaap_RelatedPartyDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_RelatedPartyDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RelatedPartyDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis" xlink:to="us-gaap_RelatedPartyDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note8RelatedPartyTransactionsDetailsTable" xlink:to="us-gaap_PropertyPlantAndEquipmentByTypeAxis" order="3" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_NatureOfExpenseAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NatureOfExpenseAxis"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note8RelatedPartyTransactionsDetailsTable" xlink:to="us-gaap_NatureOfExpenseAxis" order="4" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_InterimPeriodCostsNotAllocableDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InterimPeriodCostsNotAllocableDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_NatureOfExpenseAxis" xlink:to="us-gaap_InterimPeriodCostsNotAllocableDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_InterimPeriodCostsNotAllocableDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InterimPeriodCostsNotAllocableDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_NatureOfExpenseAxis" xlink:to="us-gaap_InterimPeriodCostsNotAllocableDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_StatementScenarioAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementScenarioAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note8RelatedPartyTransactionsDetailsTable" xlink:to="us-gaap_StatementScenarioAxis" order="5" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_EurobulkLtdMember" xlink:href="esea-20151231.xsd#esea_EurobulkLtdMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain" xlink:to="esea_EurobulkLtdMember" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_EurochartMember" xlink:href="esea-20151231.xsd#esea_EurochartMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain" xlink:to="esea_EurochartMember" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_SentinelMember" xlink:href="esea-20151231.xsd#esea_SentinelMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain" xlink:to="esea_SentinelMember" order="2" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_TechnomarMember" xlink:href="esea-20151231.xsd#esea_TechnomarMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain" xlink:to="esea_TechnomarMember" order="3" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_EuroseasLtdMember" xlink:href="esea-20151231.xsd#esea_EuroseasLtdMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_EntityDomain" xlink:to="esea_EuroseasLtdMember" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_EuromarLLCJointVentureMember" xlink:href="esea-20151231.xsd#esea_EuromarLLCJointVentureMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_EntityDomain" xlink:to="esea_EuromarLLCJointVentureMember" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_VesselManagementFeesMember" xlink:href="esea-20151231.xsd#esea_VesselManagementFeesMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyTransactionDomain" xlink:to="esea_VesselManagementFeesMember" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_VesselSalesMember" xlink:href="esea-20151231.xsd#esea_VesselSalesMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyTransactionDomain" xlink:to="esea_VesselSalesMember" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_CharterRevenuesMember" xlink:href="esea-20151231.xsd#esea_CharterRevenuesMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyTransactionDomain" xlink:to="esea_CharterRevenuesMember" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_MVEiriniPMember" xlink:href="esea-20151231.xsd#esea_MVEiriniPMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_MVEiriniPMember" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_FixedManagementFeesMember" xlink:href="esea-20151231.xsd#esea_FixedManagementFeesMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InterimPeriodCostsNotAllocableDomain" xlink:to="esea_FixedManagementFeesMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_AfterDiscountMember" xlink:href="esea-20151231.xsd#esea_AfterDiscountMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScenarioUnspecifiedDomain" xlink:to="esea_AfterDiscountMember" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioForecastMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioForecastMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScenarioUnspecifiedDomain" xlink:to="us-gaap_ScenarioForecastMember" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_ServceManagementCostsDailyFeeRelatedParty" xlink:href="esea-20151231.xsd#esea_ServceManagementCostsDailyFeeRelatedParty"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note8RelatedPartyTransactionsDetailsLineItems" xlink:to="esea_ServceManagementCostsDailyFeeRelatedParty" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_RelatedPartyTransactionAmountsOfTransaction" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RelatedPartyTransactionAmountsOfTransaction"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note8RelatedPartyTransactionsDetailsLineItems" xlink:to="us-gaap_RelatedPartyTransactionAmountsOfTransaction" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_RelatedPartyAgreementTerm" xlink:href="esea-20151231.xsd#esea_RelatedPartyAgreementTerm"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note8RelatedPartyTransactionsDetailsLineItems" xlink:to="esea_RelatedPartyAgreementTerm" order="3" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_RelatedPartyTransactionDiscountPercentage" xlink:href="esea-20151231.xsd#esea_RelatedPartyTransactionDiscountPercentage"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note8RelatedPartyTransactionsDetailsLineItems" xlink:to="esea_RelatedPartyTransactionDiscountPercentage" order="4" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_NumberOfVessels" xlink:href="esea-20151231.xsd#esea_NumberOfVessels"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note8RelatedPartyTransactionsDetailsLineItems" xlink:to="esea_NumberOfVessels" order="5" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_RelatedPartyTransactionDailyFeePerVesselPerDayInOperation" xlink:href="esea-20151231.xsd#esea_RelatedPartyTransactionDailyFeePerVesselPerDayInOperation"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note8RelatedPartyTransactionsDetailsLineItems" xlink:to="esea_RelatedPartyTransactionDailyFeePerVesselPerDayInOperation" order="6" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_RelatedPartyTransactionDailyFeePerVesselPerDayInLayUp" xlink:href="esea-20151231.xsd#esea_RelatedPartyTransactionDailyFeePerVesselPerDayInLayUp"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note8RelatedPartyTransactionsDetailsLineItems" xlink:to="esea_RelatedPartyTransactionDailyFeePerVesselPerDayInLayUp" order="7" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_DueToRelatedPartiesCurrentAndNoncurrent" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DueToRelatedPartiesCurrentAndNoncurrent"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note8RelatedPartyTransactionsDetailsLineItems" xlink:to="us-gaap_DueToRelatedPartiesCurrentAndNoncurrent" order="8" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_RelatedPartyTransactionCommissionPercentage" xlink:href="esea-20151231.xsd#esea_RelatedPartyTransactionCommissionPercentage"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note8RelatedPartyTransactionsDetailsLineItems" xlink:to="esea_RelatedPartyTransactionCommissionPercentage" order="9" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_RelatedPartyTransactionExpensesFromTransactionsWithRelatedParty" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RelatedPartyTransactionExpensesFromTransactionsWithRelatedParty"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note8RelatedPartyTransactionsDetailsLineItems" xlink:to="us-gaap_RelatedPartyTransactionExpensesFromTransactionsWithRelatedParty" order="10" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_RelatedPartyTransactionCommissionOnPremiumMaximumPercentage" xlink:href="esea-20151231.xsd#esea_RelatedPartyTransactionCommissionOnPremiumMaximumPercentage"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note8RelatedPartyTransactionsDetailsLineItems" xlink:to="esea_RelatedPartyTransactionCommissionOnPremiumMaximumPercentage" order="11" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_RelatedPartyTransactionAmountsOfTransactionPerCrewMemberPerMonth" xlink:href="esea-20151231.xsd#esea_RelatedPartyTransactionAmountsOfTransactionPerCrewMemberPerMonth"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note8RelatedPartyTransactionsDetailsLineItems" xlink:to="esea_RelatedPartyTransactionAmountsOfTransactionPerCrewMemberPerMonth" order="12" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note9LongtermDebtDetails">  
    <loc xlink:type="locator" xlink:label="esea_Note9LongtermDebtDetailsLineItems" xlink:href="esea-20151231.xsd#esea_Note9LongtermDebtDetailsLineItems"/>     
    <loc xlink:type="locator" xlink:label="esea_Note9LongtermDebtDetailsTable" xlink:href="esea-20151231.xsd#esea_Note9LongtermDebtDetailsTable"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="esea_Note9LongtermDebtDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note9LongtermDebtDetailsTable" xlink:to="us-gaap_DebtInstrumentAxis" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentNameDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DebtInstrumentAxis" xlink:to="us-gaap_DebtInstrumentNameDomain" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentNameDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DebtInstrumentAxis" xlink:to="us-gaap_DebtInstrumentNameDomain_0" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_RangeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RangeAxis"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note9LongtermDebtDetailsTable" xlink:to="us-gaap_RangeAxis" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_RangeMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RangeMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_RangeAxis" xlink:to="us-gaap_RangeMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_RangeMember_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RangeMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_RangeAxis" xlink:to="us-gaap_RangeMember_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_VariableRateAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_VariableRateAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note9LongtermDebtDetailsTable" xlink:to="us-gaap_VariableRateAxis" order="2" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_VariableRateDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_VariableRateDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_VariableRateAxis" xlink:to="us-gaap_VariableRateDomain" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_VariableRateDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_VariableRateDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_VariableRateAxis" xlink:to="us-gaap_VariableRateDomain_0" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="dei_LegalEntityAxis" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_LegalEntityAxis"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note9LongtermDebtDetailsTable" xlink:to="dei_LegalEntityAxis" order="3" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="dei_EntityDomain" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="dei_LegalEntityAxis" xlink:to="dei_EntityDomain" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="dei_EntityDomain_0" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="dei_LegalEntityAxis" xlink:to="dei_EntityDomain_0" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_TypeOfArrangementAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_TypeOfArrangementAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note9LongtermDebtDetailsTable" xlink:to="us-gaap_TypeOfArrangementAxis" order="4" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_ArrangementsAndNonarrangementTransactionsMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_TypeOfArrangementAxis" xlink:to="us-gaap_ArrangementsAndNonarrangementTransactionsMember" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ArrangementsAndNonarrangementTransactionsMember_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_TypeOfArrangementAxis" xlink:to="us-gaap_ArrangementsAndNonarrangementTransactionsMember_0" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note9LongtermDebtDetailsTable" xlink:to="us-gaap_PropertyPlantAndEquipmentByTypeAxis" order="5" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_StatementScenarioAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementScenarioAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note9LongtermDebtDetailsTable" xlink:to="us-gaap_StatementScenarioAxis" order="6" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_FirstSetOfPaymentsMember" xlink:href="esea-20151231.xsd#esea_FirstSetOfPaymentsMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_FirstSetOfPaymentsMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_SecondSetOfPaymentsMember" xlink:href="esea-20151231.xsd#esea_SecondSetOfPaymentsMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_SecondSetOfPaymentsMember" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_ThirdSetOfPaymentsMember" xlink:href="esea-20151231.xsd#esea_ThirdSetOfPaymentsMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_ThirdSetOfPaymentsMember" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_AddendumMember" xlink:href="esea-20151231.xsd#esea_AddendumMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_AddendumMember" order="3" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_RefinancingMember" xlink:href="esea-20151231.xsd#esea_RefinancingMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_RefinancingMember" order="4" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_SupplementalAgreementMember" xlink:href="esea-20151231.xsd#esea_SupplementalAgreementMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_SupplementalAgreementMember" order="5" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_FourInstallmentsMember" xlink:href="esea-20151231.xsd#esea_FourInstallmentsMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_FourInstallmentsMember" order="6" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_TwentyEightInstallmentsMember" xlink:href="esea-20151231.xsd#esea_TwentyEightInstallmentsMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_TwentyEightInstallmentsMember" order="7" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_TrancheAMember" xlink:href="esea-20151231.xsd#esea_TrancheAMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_TrancheAMember" order="8" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_TrancheBMember" xlink:href="esea-20151231.xsd#esea_TrancheBMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_TrancheBMember" order="9" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_FinanceConstructionOfHullNoDY160Member" xlink:href="esea-20151231.xsd#esea_FinanceConstructionOfHullNoDY160Member"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_FinanceConstructionOfHullNoDY160Member" order="10" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_FinanceConstructionOfHullNoDY161Member" xlink:href="esea-20151231.xsd#esea_FinanceConstructionOfHullNoDY161Member"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_FinanceConstructionOfHullNoDY161Member" order="11" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_MinimumMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_MinimumMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RangeMember" xlink:to="us-gaap_MinimumMember" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_MaximumMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_MaximumMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RangeMember" xlink:to="us-gaap_MaximumMember" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_XingangShippingLtdMember" xlink:href="esea-20151231.xsd#esea_XingangShippingLtdMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_EntityDomain" xlink:to="esea_XingangShippingLtdMember" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_ManolisShippingLtdMember" xlink:href="esea-20151231.xsd#esea_ManolisShippingLtdMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_EntityDomain" xlink:to="esea_ManolisShippingLtdMember" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_SAFConcordShippingLtdMember" xlink:href="esea-20151231.xsd#esea_SAFConcordShippingLtdMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_EntityDomain" xlink:to="esea_SAFConcordShippingLtdMember" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_PantelisShippingCorpMember" xlink:href="esea-20151231.xsd#esea_PantelisShippingCorpMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_EntityDomain" xlink:to="esea_PantelisShippingCorpMember" order="3" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_AggelikiShippingLtdMember" xlink:href="esea-20151231.xsd#esea_AggelikiShippingLtdMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_EntityDomain" xlink:to="esea_AggelikiShippingLtdMember" order="4" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_NoumeaShippingLtdMember" xlink:href="esea-20151231.xsd#esea_NoumeaShippingLtdMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_EntityDomain" xlink:to="esea_NoumeaShippingLtdMember" order="5" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_EiriniShippingLtdAndEleniShippingLtdMember" xlink:href="esea-20151231.xsd#esea_EiriniShippingLtdAndEleniShippingLtdMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_EntityDomain" xlink:to="esea_EiriniShippingLtdAndEleniShippingLtdMember" order="6" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_EuroseasLtdMember" xlink:href="esea-20151231.xsd#esea_EuroseasLtdMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_EntityDomain" xlink:to="esea_EuroseasLtdMember" order="7" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_MVEiriniAndMVEleniMember" xlink:href="esea-20151231.xsd#esea_MVEiriniAndMVEleniMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_MVEiriniAndMVEleniMember" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_LondonInterbankOfferedRateLIBORMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LondonInterbankOfferedRateLIBORMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_VariableRateDomain" xlink:to="us-gaap_LondonInterbankOfferedRateLIBORMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_JoannaMaritimeLtdAsGuarantorMember" xlink:href="esea-20151231.xsd#esea_JoannaMaritimeLtdAsGuarantorMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ArrangementsAndNonarrangementTransactionsMember" xlink:to="esea_JoannaMaritimeLtdAsGuarantorMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_AlcinoeShippingLtdAsGuarantorMember" xlink:href="esea-20151231.xsd#esea_AlcinoeShippingLtdAsGuarantorMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ArrangementsAndNonarrangementTransactionsMember" xlink:to="esea_AlcinoeShippingLtdAsGuarantorMember" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_FirstFiveYearInstallmentsMember" xlink:href="esea-20151231.xsd#esea_FirstFiveYearInstallmentsMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScenarioUnspecifiedDomain" xlink:to="esea_FirstFiveYearInstallmentsMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentFaceAmount" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentFaceAmount"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="us-gaap_DebtInstrumentFaceAmount" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_DebtInstrumentNumberOfQuarterlyPayments" xlink:href="esea-20151231.xsd#esea_DebtInstrumentNumberOfQuarterlyPayments"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="esea_DebtInstrumentNumberOfQuarterlyPayments" order="2" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentPeriodicPayment" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentPeriodicPayment"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="us-gaap_DebtInstrumentPeriodicPayment" order="3" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentPeriodicPaymentTermsBalloonPaymentToBePaid" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentPeriodicPaymentTermsBalloonPaymentToBePaid"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="us-gaap_DebtInstrumentPeriodicPaymentTermsBalloonPaymentToBePaid" order="4" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentBasisSpreadOnVariableRate1" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="us-gaap_DebtInstrumentBasisSpreadOnVariableRate1" order="5" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_DebtInstrumentMinimumCashBalance" xlink:href="esea-20151231.xsd#esea_DebtInstrumentMinimumCashBalance"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="esea_DebtInstrumentMinimumCashBalance" order="6" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ProceedsFromIssuanceOfLongTermDebt" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ProceedsFromIssuanceOfLongTermDebt"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="us-gaap_ProceedsFromIssuanceOfLongTermDebt" order="7" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ExtinguishmentOfDebtAmount" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ExtinguishmentOfDebtAmount"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="us-gaap_ExtinguishmentOfDebtAmount" order="8" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_DebtInstrumentMinimumCashBalanceCancelled" xlink:href="esea-20151231.xsd#esea_DebtInstrumentMinimumCashBalanceCancelled"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="esea_DebtInstrumentMinimumCashBalanceCancelled" order="9" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentCarryingAmount" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentCarryingAmount"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="us-gaap_DebtInstrumentCarryingAmount" order="10" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_DebtInstrumentPercentageToFairMarketValue" xlink:href="esea-20151231.xsd#esea_DebtInstrumentPercentageToFairMarketValue"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="esea_DebtInstrumentPercentageToFairMarketValue" order="11" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_DebtInstrumentNumberOfTranches" xlink:href="esea-20151231.xsd#esea_DebtInstrumentNumberOfTranches"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="esea_DebtInstrumentNumberOfTranches" order="12" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_DebtInstrumentNumberOfSemiAnnualPayments" xlink:href="esea-20151231.xsd#esea_DebtInstrumentNumberOfSemiAnnualPayments"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="esea_DebtInstrumentNumberOfSemiAnnualPayments" order="13" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_CashPledgedAsCollateralForLoanFacility" xlink:href="esea-20151231.xsd#esea_CashPledgedAsCollateralForLoanFacility"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="esea_CashPledgedAsCollateralForLoanFacility" order="14" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_PropertyPlantAndEquipmentValueToOutstandingFacilityAmount" xlink:href="esea-20151231.xsd#esea_PropertyPlantAndEquipmentValueToOutstandingFacilityAmount"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="esea_PropertyPlantAndEquipmentValueToOutstandingFacilityAmount" order="15" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_TermLoanFacilityMaximumBorrowingCapacity" xlink:href="esea-20151231.xsd#esea_TermLoanFacilityMaximumBorrowingCapacity"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="esea_TermLoanFacilityMaximumBorrowingCapacity" order="16" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_TermLoanFacilityMaximumBorrowingCapacityAsAPercentageOfVesselMarketValueUponDeliveryUnderCharter" xlink:href="esea-20151231.xsd#esea_TermLoanFacilityMaximumBorrowingCapacityAsAPercentageOfVesselMarketValueUponDeliveryUnderCharter"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="esea_TermLoanFacilityMaximumBorrowingCapacityAsAPercentageOfVesselMarketValueUponDeliveryUnderCharter" order="17" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_TermLoanFacilityMaximumBorrowingCapacityAsAPercentageOfVesselMarketValueUponDeliveryCharterFree" xlink:href="esea-20151231.xsd#esea_TermLoanFacilityMaximumBorrowingCapacityAsAPercentageOfVesselMarketValueUponDeliveryCharterFree"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="esea_TermLoanFacilityMaximumBorrowingCapacityAsAPercentageOfVesselMarketValueUponDeliveryCharterFree" order="18" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentTerm" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentTerm"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="us-gaap_DebtInstrumentTerm" order="19" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_LineOfCreditFacilityCommitmentFeePercentage" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LineOfCreditFacilityCommitmentFeePercentage"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="us-gaap_LineOfCreditFacilityCommitmentFeePercentage" order="20" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_TermLoanFacilityMaximumBorrowingCapacityAsAPercentageOfVesselMarketValueUponDelivery" xlink:href="esea-20151231.xsd#esea_TermLoanFacilityMaximumBorrowingCapacityAsAPercentageOfVesselMarketValueUponDelivery"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="esea_TermLoanFacilityMaximumBorrowingCapacityAsAPercentageOfVesselMarketValueUponDelivery" order="21" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_PropertyPlantAndEquipmentFairFalueToOustandingLoanNetRatio" xlink:href="esea-20151231.xsd#esea_PropertyPlantAndEquipmentFairFalueToOustandingLoanNetRatio"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="esea_PropertyPlantAndEquipmentFairFalueToOustandingLoanNetRatio" order="22" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_LimitedDividendsPercentageLoansToProfits" xlink:href="esea-20151231.xsd#esea_LimitedDividendsPercentageLoansToProfits"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="esea_LimitedDividendsPercentageLoansToProfits" order="23" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_RestrictedCashAndCashEquivalents" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RestrictedCashAndCashEquivalents"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="us-gaap_RestrictedCashAndCashEquivalents" order="24" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_InterestExpense" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InterestExpense"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="us-gaap_InterestExpense" order="25" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_AccumulatedCapitalizedInterestCosts" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccumulatedCapitalizedInterestCosts"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note9LongtermDebtDetailsLineItems" xlink:to="us-gaap_AccumulatedCapitalizedInterestCosts" order="26" xbrldt:closed="true"/>    
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note10IncomeTaxesDetails">  
    <loc xlink:type="locator" xlink:label="us-gaap_IncomeTaxDisclosureAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncomeTaxDisclosureAbstract"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestmentOwnershipPercentage" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestmentOwnershipPercentage"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_IncomeTaxDisclosureAbstract" xlink:to="us-gaap_EquityMethodInvestmentOwnershipPercentage" order="0" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note11CommitmentsandContingenciesDetails">   
    <loc xlink:type="locator" xlink:label="esea_Note11CommitmentsandContingenciesDetailsLineItems" xlink:href="esea-20151231.xsd#esea_Note11CommitmentsandContingenciesDetailsLineItems"/>  
    <loc xlink:type="locator" xlink:label="esea_Note11CommitmentsandContingenciesDetailsTable" xlink:href="esea-20151231.xsd#esea_Note11CommitmentsandContingenciesDetailsTable"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note11CommitmentsandContingenciesDetailsLineItems" xlink:to="esea_Note11CommitmentsandContingenciesDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note11CommitmentsandContingenciesDetailsTable" xlink:to="us-gaap_PropertyPlantAndEquipmentByTypeAxis" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_SubsequentEventTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SubsequentEventTypeAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note11CommitmentsandContingenciesDetailsTable" xlink:to="us-gaap_SubsequentEventTypeAxis" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_SubsequentEventTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_SubsequentEventTypeAxis" xlink:to="us-gaap_SubsequentEventTypeDomain" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_SubsequentEventTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_SubsequentEventTypeAxis" xlink:to="us-gaap_SubsequentEventTypeDomain_0" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_SubsequentEventMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SubsequentEventMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventTypeDomain" xlink:to="us-gaap_SubsequentEventMember" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_OneVesselMember" xlink:href="esea-20151231.xsd#esea_OneVesselMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_OneVesselMember" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_NumberOfVesselsUnderConstruction" xlink:href="esea-20151231.xsd#esea_NumberOfVesselsUnderConstruction"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note11CommitmentsandContingenciesDetailsLineItems" xlink:to="esea_NumberOfVesselsUnderConstruction" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_NumberOfVesselsDelivered" xlink:href="esea-20151231.xsd#esea_NumberOfVesselsDelivered"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note11CommitmentsandContingenciesDetailsLineItems" xlink:to="esea_NumberOfVesselsDelivered" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_PaymentsToAcquirePropertyPlantAndEquipment" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note11CommitmentsandContingenciesDetailsLineItems" xlink:to="us-gaap_PaymentsToAcquirePropertyPlantAndEquipment" order="3" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PurchaseObligationDueInNextTwelveMonths" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PurchaseObligationDueInNextTwelveMonths"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note11CommitmentsandContingenciesDetailsLineItems" xlink:to="us-gaap_PurchaseObligationDueInNextTwelveMonths" order="4" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_PurchaseObligationDueInSecondYear" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PurchaseObligationDueInSecondYear"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note11CommitmentsandContingenciesDetailsLineItems" xlink:to="us-gaap_PurchaseObligationDueInSecondYear" order="5" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PurchaseObligationDueInThirdYear" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PurchaseObligationDueInThirdYear"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note11CommitmentsandContingenciesDetailsLineItems" xlink:to="us-gaap_PurchaseObligationDueInThirdYear" order="6" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note12StockIncentivePlanDetails">    
    <loc xlink:type="locator" xlink:label="esea_Note12StockIncentivePlanDetailsLineItems" xlink:href="esea-20151231.xsd#esea_Note12StockIncentivePlanDetailsLineItems"/>    
    <loc xlink:type="locator" xlink:label="esea_Note12StockIncentivePlanDetailsTable" xlink:href="esea-20151231.xsd#esea_Note12StockIncentivePlanDetailsTable"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note12StockIncentivePlanDetailsLineItems" xlink:to="esea_Note12StockIncentivePlanDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_AwardTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AwardTypeAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note12StockIncentivePlanDetailsTable" xlink:to="us-gaap_AwardTypeAxis" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_AwardTypeAxis" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_AwardTypeAxis" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_0" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_VestingAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_VestingAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note12StockIncentivePlanDetailsTable" xlink:to="us-gaap_VestingAxis" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_VestingDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_VestingDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_VestingAxis" xlink:to="us-gaap_VestingDomain" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_VestingDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_VestingDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_VestingAxis" xlink:to="us-gaap_VestingDomain_0" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_TitleOfIndividualAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_TitleOfIndividualAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note12StockIncentivePlanDetailsTable" xlink:to="us-gaap_TitleOfIndividualAxis" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_TitleOfIndividualWithRelationshipToEntityDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_TitleOfIndividualWithRelationshipToEntityDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_TitleOfIndividualAxis" xlink:to="us-gaap_TitleOfIndividualWithRelationshipToEntityDomain" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_TitleOfIndividualWithRelationshipToEntityDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_TitleOfIndividualWithRelationshipToEntityDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_TitleOfIndividualAxis" xlink:to="us-gaap_TitleOfIndividualWithRelationshipToEntityDomain_0" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PlanNameAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PlanNameAxis"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note12StockIncentivePlanDetailsTable" xlink:to="us-gaap_PlanNameAxis" order="3" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PlanNameDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PlanNameDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PlanNameAxis" xlink:to="us-gaap_PlanNameDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_PlanNameDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PlanNameDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PlanNameAxis" xlink:to="us-gaap_PlanNameDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_The19KeyPersonsMember" xlink:href="esea-20151231.xsd#esea_The19KeyPersonsMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_TitleOfIndividualWithRelationshipToEntityDomain" xlink:to="esea_The19KeyPersonsMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_OfficersAndDirectorsMember" xlink:href="esea-20151231.xsd#esea_OfficersAndDirectorsMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_TitleOfIndividualWithRelationshipToEntityDomain" xlink:to="esea_OfficersAndDirectorsMember" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_EurobulkEmployeesMember" xlink:href="esea-20151231.xsd#esea_EurobulkEmployeesMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_TitleOfIndividualWithRelationshipToEntityDomain" xlink:to="esea_EurobulkEmployeesMember" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_The2010PlanMember" xlink:href="esea-20151231.xsd#esea_The2010PlanMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PlanNameDomain" xlink:to="esea_The2010PlanMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_The2014PlanMember" xlink:href="esea-20151231.xsd#esea_The2014PlanMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PlanNameDomain" xlink:to="esea_The2014PlanMember" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_VestingOnJuly12014Member" xlink:href="esea-20151231.xsd#esea_VestingOnJuly12014Member"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_VestingDomain" xlink:to="esea_VestingOnJuly12014Member" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_VestingOnJuly12015Member" xlink:href="esea-20151231.xsd#esea_VestingOnJuly12015Member"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_VestingDomain" xlink:to="esea_VestingOnJuly12015Member" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_VestingNovember162015Member" xlink:href="esea-20151231.xsd#esea_VestingNovember162015Member"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_VestingDomain" xlink:to="esea_VestingNovember162015Member" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_VestingNovember162016Member" xlink:href="esea-20151231.xsd#esea_VestingNovember162016Member"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_VestingDomain" xlink:to="esea_VestingNovember162016Member" order="3" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_VestingOnJuly12016Member" xlink:href="esea-20151231.xsd#esea_VestingOnJuly12016Member"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_VestingDomain" xlink:to="esea_VestingOnJuly12016Member" order="4" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_VestingOnJuly12017Member" xlink:href="esea-20151231.xsd#esea_VestingOnJuly12017Member"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_VestingDomain" xlink:to="esea_VestingOnJuly12017Member" order="5" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_RestrictedStockMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RestrictedStockMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:to="us-gaap_RestrictedStockMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note12StockIncentivePlanDetailsLineItems" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_ShareBasedCompensationArrangementByShareBasedPaymentAwardedTerm" xlink:href="esea-20151231.xsd#esea_ShareBasedCompensationArrangementByShareBasedPaymentAwardedTerm"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note12StockIncentivePlanDetailsLineItems" xlink:to="esea_ShareBasedCompensationArrangementByShareBasedPaymentAwardedTerm" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note12StockIncentivePlanDetailsLineItems" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod" order="3" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_NumberOfKeyPeopleIssuedAwards" xlink:href="esea-20151231.xsd#esea_NumberOfKeyPeopleIssuedAwards"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note12StockIncentivePlanDetailsLineItems" xlink:to="esea_NumberOfKeyPeopleIssuedAwards" order="4" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardAwardVestingRightsPercentage" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardAwardVestingRightsPercentage"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note12StockIncentivePlanDetailsLineItems" xlink:to="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardAwardVestingRightsPercentage" order="5" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_AllocatedShareBasedCompensationExpense" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note12StockIncentivePlanDetailsLineItems" xlink:to="us-gaap_AllocatedShareBasedCompensationExpense" order="6" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note12StockIncentivePlanDetailsLineItems" xlink:to="us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized" order="7" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note12StockIncentivePlanDetailsLineItems" xlink:to="us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1" order="8" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodTotalFairValue" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodTotalFairValue"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note12StockIncentivePlanDetailsLineItems" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodTotalFairValue" order="9" xbrldt:closed="true"/>   
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note13EarningsLossPerShareDetails"> 
    <loc xlink:type="locator" xlink:label="us-gaap_EarningsPerShareAbstract" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EarningsPerShareAbstract"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EarningsPerShareAbstract" xlink:to="us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount" order="0" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note15FinancialInstrumentsDetails"> 
    <loc xlink:type="locator" xlink:label="esea_Note15FinancialInstrumentsDetailsLineItems" xlink:href="esea-20151231.xsd#esea_Note15FinancialInstrumentsDetailsLineItems"/>   
    <loc xlink:type="locator" xlink:label="esea_Note15FinancialInstrumentsDetailsTable" xlink:href="esea-20151231.xsd#esea_Note15FinancialInstrumentsDetailsTable"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note15FinancialInstrumentsDetailsLineItems" xlink:to="esea_Note15FinancialInstrumentsDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note15FinancialInstrumentsDetailsTable" xlink:to="us-gaap_PropertyPlantAndEquipmentByTypeAxis" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInstrumentRiskAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note15FinancialInstrumentsDetailsTable" xlink:to="us-gaap_DerivativeInstrumentRiskAxis" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeContractTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DerivativeInstrumentRiskAxis" xlink:to="us-gaap_DerivativeContractTypeDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeContractTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DerivativeInstrumentRiskAxis" xlink:to="us-gaap_DerivativeContractTypeDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_InterestRateSwapMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InterestRateSwapMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain" xlink:to="us-gaap_InterestRateSwapMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_MVAristidesMember" xlink:href="esea-20151231.xsd#esea_MVAristidesMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_MVAristidesMember" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeNumberOfInstrumentsHeld" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeNumberOfInstrumentsHeld"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note15FinancialInstrumentsDetailsLineItems" xlink:to="us-gaap_DerivativeNumberOfInstrumentsHeld" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="invest_DerivativeNotionalAmount" xlink:href="http://xbrl.sec.gov/invest/2013/invest-2013-01-31.xsd#invest_DerivativeNotionalAmount"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note15FinancialInstrumentsDetailsLineItems" xlink:to="invest_DerivativeNotionalAmount" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_AssetImpairmentCharges" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AssetImpairmentCharges"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note15FinancialInstrumentsDetailsLineItems" xlink:to="us-gaap_AssetImpairmentCharges" order="3" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DisposalGroupIncludingDiscontinuedOperationConsideration" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DisposalGroupIncludingDiscontinuedOperationConsideration"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note15FinancialInstrumentsDetailsLineItems" xlink:to="us-gaap_DisposalGroupIncludingDiscontinuedOperationConsideration" order="4" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_LongTermDebtFairValue" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LongTermDebtFairValue"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note15FinancialInstrumentsDetailsLineItems" xlink:to="us-gaap_LongTermDebtFairValue" order="5" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_DifferencebetweenFairValueandCarryingValue" xlink:href="esea-20151231.xsd#esea_DifferencebetweenFairValueandCarryingValue"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note15FinancialInstrumentsDetailsLineItems" xlink:to="esea_DifferencebetweenFairValueandCarryingValue" order="6" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_LongTermDebt" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LongTermDebt"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note15FinancialInstrumentsDetailsLineItems" xlink:to="us-gaap_LongTermDebt" order="7" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_FairValueAssumptionsExpectedDividendRate" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_FairValueAssumptionsExpectedDividendRate"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note15FinancialInstrumentsDetailsLineItems" xlink:to="us-gaap_FairValueAssumptionsExpectedDividendRate" order="8" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_OtherInvestmentEffectOfOnePercentagePointIncreaseDecreaseOnFairValue" xlink:href="esea-20151231.xsd#esea_OtherInvestmentEffectOfOnePercentagePointIncreaseDecreaseOnFairValue"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note15FinancialInstrumentsDetailsLineItems" xlink:to="esea_OtherInvestmentEffectOfOnePercentagePointIncreaseDecreaseOnFairValue" order="9" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note16DerivativeFinancialInstrumentsDetails"> 
    <loc xlink:type="locator" xlink:label="esea_Note16DerivativeFinancialInstrumentsDetailsLineItems" xlink:href="esea-20151231.xsd#esea_Note16DerivativeFinancialInstrumentsDetailsLineItems"/>   
    <loc xlink:type="locator" xlink:label="esea_Note16DerivativeFinancialInstrumentsDetailsTable" xlink:href="esea-20151231.xsd#esea_Note16DerivativeFinancialInstrumentsDetailsTable"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note16DerivativeFinancialInstrumentsDetailsLineItems" xlink:to="esea_Note16DerivativeFinancialInstrumentsDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeInstrumentRiskAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note16DerivativeFinancialInstrumentsDetailsTable" xlink:to="us-gaap_DerivativeInstrumentRiskAxis" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeContractTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DerivativeInstrumentRiskAxis" xlink:to="us-gaap_DerivativeContractTypeDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeContractTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DerivativeInstrumentRiskAxis" xlink:to="us-gaap_DerivativeContractTypeDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_StatementScenarioAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementScenarioAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note16DerivativeFinancialInstrumentsDetailsTable" xlink:to="us-gaap_StatementScenarioAxis" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_CounterpartyNameAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CounterpartyNameAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note16DerivativeFinancialInstrumentsDetailsTable" xlink:to="us-gaap_CounterpartyNameAxis" order="2" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_RepurchaseAgreementCounterpartyNameDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RepurchaseAgreementCounterpartyNameDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_CounterpartyNameAxis" xlink:to="us-gaap_RepurchaseAgreementCounterpartyNameDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_RepurchaseAgreementCounterpartyNameDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RepurchaseAgreementCounterpartyNameDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_CounterpartyNameAxis" xlink:to="us-gaap_RepurchaseAgreementCounterpartyNameDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_InterestRateSwapMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InterestRateSwapMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain" xlink:to="us-gaap_InterestRateSwapMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_FirstTwoContractMember" xlink:href="esea-20151231.xsd#esea_FirstTwoContractMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain" xlink:to="esea_FirstTwoContractMember" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_InterestRateSwapOneMember" xlink:href="esea-20151231.xsd#esea_InterestRateSwapOneMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain" xlink:to="esea_InterestRateSwapOneMember" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_InterestRateSwapTwoMember" xlink:href="esea-20151231.xsd#esea_InterestRateSwapTwoMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain" xlink:to="esea_InterestRateSwapTwoMember" order="3" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_ForwardStepUpSwapMember" xlink:href="esea-20151231.xsd#esea_ForwardStepUpSwapMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain" xlink:to="esea_ForwardStepUpSwapMember" order="4" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_November282016Member" xlink:href="esea-20151231.xsd#esea_November282016Member"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScenarioUnspecifiedDomain" xlink:to="esea_November282016Member" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_November282017Member" xlink:href="esea-20151231.xsd#esea_November282017Member"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScenarioUnspecifiedDomain" xlink:to="esea_November282017Member" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_May282019Member" xlink:href="esea-20151231.xsd#esea_May282019Member"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScenarioUnspecifiedDomain" xlink:to="esea_May282019Member" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_EurobankMember" xlink:href="esea-20151231.xsd#esea_EurobankMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RepurchaseAgreementCounterpartyNameDomain" xlink:to="esea_EurobankMember" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeNumberOfInstrumentsHeld" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeNumberOfInstrumentsHeld"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note16DerivativeFinancialInstrumentsDetailsLineItems" xlink:to="us-gaap_DerivativeNumberOfInstrumentsHeld" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="invest_DerivativeNotionalAmount" xlink:href="http://xbrl.sec.gov/invest/2013/invest-2013-01-31.xsd#invest_DerivativeNotionalAmount"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note16DerivativeFinancialInstrumentsDetailsLineItems" xlink:to="invest_DerivativeNotionalAmount" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeFixedInterestRate" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeFixedInterestRate"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note16DerivativeFinancialInstrumentsDetailsLineItems" xlink:to="us-gaap_DerivativeFixedInterestRate" order="3" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DerivativeAverageFixedInterestRate" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DerivativeAverageFixedInterestRate"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note16DerivativeFinancialInstrumentsDetailsLineItems" xlink:to="us-gaap_DerivativeAverageFixedInterestRate" order="4" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note17InvestmentinJointVentureandOtherInvestmentDetails">   
    <loc xlink:type="locator" xlink:label="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:href="esea-20151231.xsd#esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems"/>  
    <loc xlink:type="locator" xlink:label="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsTable" xlink:href="esea-20151231.xsd#esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsTable"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsTable" xlink:to="us-gaap_DebtInstrumentAxis" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentNameDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DebtInstrumentAxis" xlink:to="us-gaap_DebtInstrumentNameDomain" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentNameDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DebtInstrumentAxis" xlink:to="us-gaap_DebtInstrumentNameDomain_0" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsTable" xlink:to="us-gaap_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvesteeNameDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvesteeNameDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis" xlink:to="us-gaap_EquityMethodInvesteeNameDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvesteeNameDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvesteeNameDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis" xlink:to="us-gaap_EquityMethodInvesteeNameDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="dei_LegalEntityAxis" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_LegalEntityAxis"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsTable" xlink:to="dei_LegalEntityAxis" order="2" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="dei_EntityDomain" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="dei_LegalEntityAxis" xlink:to="dei_EntityDomain" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="dei_EntityDomain_0" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="dei_LegalEntityAxis" xlink:to="dei_EntityDomain_0" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_CounterpartyNameAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CounterpartyNameAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsTable" xlink:to="us-gaap_CounterpartyNameAxis" order="3" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_RepurchaseAgreementCounterpartyNameDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RepurchaseAgreementCounterpartyNameDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_CounterpartyNameAxis" xlink:to="us-gaap_RepurchaseAgreementCounterpartyNameDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_RepurchaseAgreementCounterpartyNameDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RepurchaseAgreementCounterpartyNameDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_CounterpartyNameAxis" xlink:to="us-gaap_RepurchaseAgreementCounterpartyNameDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_CreditFacilityMaturingAugust2016Member" xlink:href="esea-20151231.xsd#esea_CreditFacilityMaturingAugust2016Member"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_CreditFacilityMaturingAugust2016Member" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_CreditFacilityMaturingOctober2016Member" xlink:href="esea-20151231.xsd#esea_CreditFacilityMaturingOctober2016Member"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain" xlink:to="esea_CreditFacilityMaturingOctober2016Member" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_CorporateJointVentureMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CorporateJointVentureMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_EntityDomain" xlink:to="us-gaap_CorporateJointVentureMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_EtonParkAndRhoneMember" xlink:href="esea-20151231.xsd#esea_EtonParkAndRhoneMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RepurchaseAgreementCounterpartyNameDomain" xlink:to="esea_EtonParkAndRhoneMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_EuromarLLCTheJointVentureMember" xlink:href="esea-20151231.xsd#esea_EuromarLLCTheJointVentureMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RepurchaseAgreementCounterpartyNameDomain" xlink:to="esea_EuromarLLCTheJointVentureMember" order="1" xbrldt:closed="true"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityMethodInvesteeNameDomain" xlink:to="esea_EuromarLLCTheJointVentureMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestmentAggregateCost" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestmentAggregateCost"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="us-gaap_EquityMethodInvestmentAggregateCost" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_EquityMethodInvestmentOwnershipPercentageMaximumPercentage" xlink:href="esea-20151231.xsd#esea_EquityMethodInvestmentOwnershipPercentageMaximumPercentage"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="esea_EquityMethodInvestmentOwnershipPercentageMaximumPercentage" order="2" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_InvestmentsinandAdvancetoAffiliatesSubsidiariesAssociatesandJointVenturesMaximumInvestment" xlink:href="esea-20151231.xsd#esea_InvestmentsinandAdvancetoAffiliatesSubsidiariesAssociatesandJointVenturesMaximumInvestment"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="esea_InvestmentsinandAdvancetoAffiliatesSubsidiariesAssociatesandJointVenturesMaximumInvestment" order="3" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_InvestmentinJointVentureTotalMaximumInvestmentbyMembers" xlink:href="esea-20151231.xsd#esea_InvestmentinJointVentureTotalMaximumInvestmentbyMembers"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="esea_InvestmentinJointVentureTotalMaximumInvestmentbyMembers" order="4" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_ConversionRatioCompanyMarketValueMultiplier" xlink:href="esea-20151231.xsd#esea_ConversionRatioCompanyMarketValueMultiplier"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="esea_ConversionRatioCompanyMarketValueMultiplier" order="5" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_IncomeLossFromEquityMethodInvestments" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncomeLossFromEquityMethodInvestments"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="us-gaap_IncomeLossFromEquityMethodInvestments" order="6" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PaymentsToAcquireEquityMethodInvestments" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PaymentsToAcquireEquityMethodInvestments"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="us-gaap_PaymentsToAcquireEquityMethodInvestments" order="7" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_EquityMethodInvestmentincreaseinmaximuminvestment" xlink:href="esea-20151231.xsd#esea_EquityMethodInvestmentincreaseinmaximuminvestment"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="esea_EquityMethodInvestmentincreaseinmaximuminvestment" order="8" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_EquityMethodInvestments" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EquityMethodInvestments"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="us-gaap_EquityMethodInvestments" order="9" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_EscrowDeposit" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_EscrowDeposit"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="us-gaap_EscrowDeposit" order="10" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_LimitedLiabilityCompanyLLCAmountOfEscrowedCashExchangedForEachPreferredUnit" xlink:href="esea-20151231.xsd#esea_LimitedLiabilityCompanyLLCAmountOfEscrowedCashExchangedForEachPreferredUnit"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="esea_LimitedLiabilityCompanyLLCAmountOfEscrowedCashExchangedForEachPreferredUnit" order="11" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_LimitedLiabilityCompanyLLCPreferredUnitIssued" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LimitedLiabilityCompanyLLCPreferredUnitIssued"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="us-gaap_LimitedLiabilityCompanyLLCPreferredUnitIssued" order="12" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_PreferredStockDividendRatePercentage" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockDividendRatePercentage"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="us-gaap_PreferredStockDividendRatePercentage" order="13" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_InvestmentIncomeDividend" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InvestmentIncomeDividend"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="us-gaap_InvestmentIncomeDividend" order="14" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_LongTermDebt" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LongTermDebt"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note17InvestmentinJointVentureandOtherInvestmentDetailsLineItems" xlink:to="us-gaap_LongTermDebt" order="15" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note18PreferredsharesDetails"> 
    <loc xlink:type="locator" xlink:label="esea_Note18PreferredsharesDetailsLineItems" xlink:href="esea-20151231.xsd#esea_Note18PreferredsharesDetailsLineItems"/>   
    <loc xlink:type="locator" xlink:label="esea_Note18PreferredsharesDetailsTable" xlink:href="esea-20151231.xsd#esea_Note18PreferredsharesDetailsTable"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note18PreferredsharesDetailsLineItems" xlink:to="esea_Note18PreferredsharesDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_RangeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RangeAxis"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note18PreferredsharesDetailsTable" xlink:to="us-gaap_RangeAxis" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_RangeMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RangeMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_RangeAxis" xlink:to="us-gaap_RangeMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_RangeMember_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RangeMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_RangeAxis" xlink:to="us-gaap_RangeMember_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_StatementClassOfStockAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note18PreferredsharesDetailsTable" xlink:to="us-gaap_StatementClassOfStockAxis" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ClassOfStockDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ClassOfStockDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementClassOfStockAxis" xlink:to="us-gaap_ClassOfStockDomain" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ClassOfStockDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ClassOfStockDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementClassOfStockAxis" xlink:to="us-gaap_ClassOfStockDomain_0" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_StatementScenarioAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementScenarioAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note18PreferredsharesDetailsTable" xlink:to="us-gaap_StatementScenarioAxis" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_CounterpartyNameAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CounterpartyNameAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note18PreferredsharesDetailsTable" xlink:to="us-gaap_CounterpartyNameAxis" order="3" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_RepurchaseAgreementCounterpartyNameDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RepurchaseAgreementCounterpartyNameDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_CounterpartyNameAxis" xlink:to="us-gaap_RepurchaseAgreementCounterpartyNameDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_RepurchaseAgreementCounterpartyNameDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RepurchaseAgreementCounterpartyNameDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_CounterpartyNameAxis" xlink:to="us-gaap_RepurchaseAgreementCounterpartyNameDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_MinimumMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_MinimumMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RangeMember" xlink:to="us-gaap_MinimumMember" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_MaximumMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_MaximumMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RangeMember" xlink:to="us-gaap_MaximumMember" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_FirstFiveYearsMember" xlink:href="esea-20151231.xsd#esea_FirstFiveYearsMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScenarioUnspecifiedDomain" xlink:to="esea_FirstFiveYearsMember" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_AfterFirstFiveYearsMember" xlink:href="esea-20151231.xsd#esea_AfterFirstFiveYearsMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScenarioUnspecifiedDomain" xlink:to="esea_AfterFirstFiveYearsMember" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_YearsSixAndSevenMember" xlink:href="esea-20151231.xsd#esea_YearsSixAndSevenMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScenarioUnspecifiedDomain" xlink:to="esea_YearsSixAndSevenMember" order="2" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_AfterYearSevenMember" xlink:href="esea-20151231.xsd#esea_AfterYearSevenMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScenarioUnspecifiedDomain" xlink:to="esea_AfterYearSevenMember" order="3" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioForecastMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioForecastMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScenarioUnspecifiedDomain" xlink:to="us-gaap_ScenarioForecastMember" order="4" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_TCPMember" xlink:href="esea-20151231.xsd#esea_TCPMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RepurchaseAgreementCounterpartyNameDomain" xlink:to="esea_TCPMember" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="esea_PreferredFriendsInvestmentCompanyMember" xlink:href="esea-20151231.xsd#esea_PreferredFriendsInvestmentCompanyMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RepurchaseAgreementCounterpartyNameDomain" xlink:to="esea_PreferredFriendsInvestmentCompanyMember" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_SeriesBPreferredStockMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SeriesBPreferredStockMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain" xlink:to="us-gaap_SeriesBPreferredStockMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_StockIssuedDuringPeriodSharesNewIssues" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsLineItems" xlink:to="us-gaap_StockIssuedDuringPeriodSharesNewIssues" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ProceedsFromIssuanceOfConvertiblePreferredStock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ProceedsFromIssuanceOfConvertiblePreferredStock"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsLineItems" xlink:to="us-gaap_ProceedsFromIssuanceOfConvertiblePreferredStock" order="2" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PreferredStockRedemptionPricePerShare" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockRedemptionPricePerShare"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsLineItems" xlink:to="us-gaap_PreferredStockRedemptionPricePerShare" order="3" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_PreferredStockDividendRatePercentage" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockDividendRatePercentage"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsLineItems" xlink:to="us-gaap_PreferredStockDividendRatePercentage" order="4" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_PreferredStockAdditionalCashDividendUnderSpecifiedConditionsPercentage" xlink:href="esea-20151231.xsd#esea_PreferredStockAdditionalCashDividendUnderSpecifiedConditionsPercentage"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsLineItems" xlink:to="esea_PreferredStockAdditionalCashDividendUnderSpecifiedConditionsPercentage" order="5" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_PreferredStockAdditionalCashDividendUnderOtherSpecifiedConditionsPercentage" xlink:href="esea-20151231.xsd#esea_PreferredStockAdditionalCashDividendUnderOtherSpecifiedConditionsPercentage"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsLineItems" xlink:to="esea_PreferredStockAdditionalCashDividendUnderOtherSpecifiedConditionsPercentage" order="6" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_PreferredStockDividendRateUnderOtherSpecifiedConditionsPercentage" xlink:href="esea-20151231.xsd#esea_PreferredStockDividendRateUnderOtherSpecifiedConditionsPercentage"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsLineItems" xlink:to="esea_PreferredStockDividendRateUnderOtherSpecifiedConditionsPercentage" order="7" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_PreferredStockConvertibleInitialConversionPrice" xlink:href="esea-20151231.xsd#esea_PreferredStockConvertibleInitialConversionPrice"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsLineItems" xlink:to="esea_PreferredStockConvertibleInitialConversionPrice" order="8" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PreferredStockRedemptionAmount" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockRedemptionAmount"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsLineItems" xlink:to="us-gaap_PreferredStockRedemptionAmount" order="9" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_IncreaseInCarryingAmountOfRedeemablePreferredStock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncreaseInCarryingAmountOfRedeemablePreferredStock"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsLineItems" xlink:to="us-gaap_IncreaseInCarryingAmountOfRedeemablePreferredStock" order="10" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_PreferredStockPercentageOfVotingRightsNumberOfCommonShares" xlink:href="esea-20151231.xsd#esea_PreferredStockPercentageOfVotingRightsNumberOfCommonShares"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsLineItems" xlink:to="esea_PreferredStockPercentageOfVotingRightsNumberOfCommonShares" order="11" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_NumberOfConsecutiveInKindDividendsDeclared" xlink:href="esea-20151231.xsd#esea_NumberOfConsecutiveInKindDividendsDeclared"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsLineItems" xlink:to="esea_NumberOfConsecutiveInKindDividendsDeclared" order="12" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DividendsPaidinkind" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DividendsPaidinkind"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note18PreferredsharesDetailsLineItems" xlink:to="us-gaap_DividendsPaidinkind" order="13" xbrldt:closed="true"/>  
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note19CommonStockDetails">  
    <loc xlink:type="locator" xlink:label="esea_Note19CommonStockDetailsLineItems" xlink:href="esea-20151231.xsd#esea_Note19CommonStockDetailsLineItems"/>     
    <loc xlink:type="locator" xlink:label="esea_Note19CommonStockDetailsTable" xlink:href="esea-20151231.xsd#esea_Note19CommonStockDetailsTable"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note19CommonStockDetailsLineItems" xlink:to="esea_Note19CommonStockDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_NonmonetaryTransactionTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NonmonetaryTransactionTypeAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note19CommonStockDetailsTable" xlink:to="us-gaap_NonmonetaryTransactionTypeAxis" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_NonmonetaryTransactionTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NonmonetaryTransactionTypeDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_NonmonetaryTransactionTypeAxis" xlink:to="us-gaap_NonmonetaryTransactionTypeDomain" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_NonmonetaryTransactionTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NonmonetaryTransactionTypeDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_NonmonetaryTransactionTypeAxis" xlink:to="us-gaap_NonmonetaryTransactionTypeDomain_0" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_ReverseStockSplitMember" xlink:href="esea-20151231.xsd#esea_ReverseStockSplitMember"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_NonmonetaryTransactionTypeDomain" xlink:to="esea_ReverseStockSplitMember" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_StockIssuedDuringPeriodSharesNewIssues" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note19CommonStockDetailsLineItems" xlink:to="us-gaap_StockIssuedDuringPeriodSharesNewIssues" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_SharePrice" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SharePrice"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note19CommonStockDetailsLineItems" xlink:to="us-gaap_SharePrice" order="2" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_ProceedsFromIssuanceOfPrivatePlacement" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ProceedsFromIssuanceOfPrivatePlacement"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note19CommonStockDetailsLineItems" xlink:to="us-gaap_ProceedsFromIssuanceOfPrivatePlacement" order="3" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_StockholdersEquityNoteStockSplitConversionRatio1" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockholdersEquityNoteStockSplitConversionRatio1"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note19CommonStockDetailsLineItems" xlink:to="us-gaap_StockholdersEquityNoteStockSplitConversionRatio1" order="4" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_ProceedsFromIssuanceOfCommonStock" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ProceedsFromIssuanceOfCommonStock"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note19CommonStockDetailsLineItems" xlink:to="us-gaap_ProceedsFromIssuanceOfCommonStock" order="5" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/Note20SubsequentEventsDetails">  
    <loc xlink:type="locator" xlink:label="esea_Note20SubsequentEventsDetailsLineItems" xlink:href="esea-20151231.xsd#esea_Note20SubsequentEventsDetailsLineItems"/>     
    <loc xlink:type="locator" xlink:label="esea_Note20SubsequentEventsDetailsTable" xlink:href="esea-20151231.xsd#esea_Note20SubsequentEventsDetailsTable"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="esea_Note20SubsequentEventsDetailsLineItems" xlink:to="esea_Note20SubsequentEventsDetailsTable" order="0" xbrldt:contextElement="segment" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note20SubsequentEventsDetailsTable" xlink:to="us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsAxis" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsAxis" xlink:to="us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsAxis" xlink:to="us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_0" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_LineOfCreditFacilityAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LineOfCreditFacilityAxis"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note20SubsequentEventsDetailsTable" xlink:to="us-gaap_LineOfCreditFacilityAxis" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_LineOfCreditFacilityLenderDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LineOfCreditFacilityLenderDomain"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_LineOfCreditFacilityAxis" xlink:to="us-gaap_LineOfCreditFacilityLenderDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_LineOfCreditFacilityLenderDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LineOfCreditFacilityLenderDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_LineOfCreditFacilityAxis" xlink:to="us-gaap_LineOfCreditFacilityLenderDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_VariableRateAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_VariableRateAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note20SubsequentEventsDetailsTable" xlink:to="us-gaap_VariableRateAxis" order="2" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_VariableRateDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_VariableRateDomain"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_VariableRateAxis" xlink:to="us-gaap_VariableRateDomain" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_VariableRateDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_VariableRateDomain"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_VariableRateAxis" xlink:to="us-gaap_VariableRateDomain_0" order="1" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note20SubsequentEventsDetailsTable" xlink:to="us-gaap_PropertyPlantAndEquipmentByTypeAxis" order="3" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_0" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_SubsequentEventTypeAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SubsequentEventTypeAxis"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note20SubsequentEventsDetailsTable" xlink:to="us-gaap_SubsequentEventTypeAxis" order="4" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_SubsequentEventTypeDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_SubsequentEventTypeAxis" xlink:to="us-gaap_SubsequentEventTypeDomain" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_SubsequentEventTypeDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_SubsequentEventTypeAxis" xlink:to="us-gaap_SubsequentEventTypeDomain_0" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_StatementScenarioAxis" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StatementScenarioAxis"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="esea_Note20SubsequentEventsDetailsTable" xlink:to="us-gaap_StatementScenarioAxis" order="5" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain" order="0" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioUnspecifiedDomain_0" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioUnspecifiedDomain"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementScenarioAxis" xlink:to="us-gaap_ScenarioUnspecifiedDomain_0" order="1" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_SubsequentEventMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_SubsequentEventMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventTypeDomain" xlink:to="us-gaap_SubsequentEventMember" order="0" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="esea_MVCaptainCostasMember" xlink:href="esea-20151231.xsd#esea_MVCaptainCostasMember"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:to="esea_MVCaptainCostasMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="us-gaap_LondonInterbankOfferedRateLIBORMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LondonInterbankOfferedRateLIBORMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_VariableRateDomain" xlink:to="us-gaap_LondonInterbankOfferedRateLIBORMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="us-gaap_CashMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CashMember"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember" xlink:to="us-gaap_CashMember" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_EurobankMember" xlink:href="esea-20151231.xsd#esea_EurobankMember"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LineOfCreditFacilityLenderDomain" xlink:to="esea_EurobankMember" order="0" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="esea_NordLBMember" xlink:href="esea-20151231.xsd#esea_NordLBMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LineOfCreditFacilityLenderDomain" xlink:to="esea_NordLBMember" order="1" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_ScenarioForecastMember" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ScenarioForecastMember"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScenarioUnspecifiedDomain" xlink:to="us-gaap_ScenarioForecastMember" order="0" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ProceedsFromIssuanceOfLongTermDebt" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ProceedsFromIssuanceOfLongTermDebt"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note20SubsequentEventsDetailsLineItems" xlink:to="us-gaap_ProceedsFromIssuanceOfLongTermDebt" order="1" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="esea_DebtInstrumentNumberOfQuarterlyPayments" xlink:href="esea-20151231.xsd#esea_DebtInstrumentNumberOfQuarterlyPayments"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note20SubsequentEventsDetailsLineItems" xlink:to="esea_DebtInstrumentNumberOfQuarterlyPayments" order="2" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentPeriodicPayment" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentPeriodicPayment"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note20SubsequentEventsDetailsLineItems" xlink:to="us-gaap_DebtInstrumentPeriodicPayment" order="3" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentPeriodicPaymentTermsBalloonPaymentToBePaid" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentPeriodicPaymentTermsBalloonPaymentToBePaid"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note20SubsequentEventsDetailsLineItems" xlink:to="us-gaap_DebtInstrumentPeriodicPaymentTermsBalloonPaymentToBePaid" order="4" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="us-gaap_DebtInstrumentBasisSpreadOnVariableRate1" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note20SubsequentEventsDetailsLineItems" xlink:to="us-gaap_DebtInstrumentBasisSpreadOnVariableRate1" order="5" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_CashPledgedAsCollateralForLoanFacility" xlink:href="esea-20151231.xsd#esea_CashPledgedAsCollateralForLoanFacility"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note20SubsequentEventsDetailsLineItems" xlink:to="esea_CashPledgedAsCollateralForLoanFacility" order="6" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="esea_DebtInstrumentNumberOfSemiAnnualPayments" xlink:href="esea-20151231.xsd#esea_DebtInstrumentNumberOfSemiAnnualPayments"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note20SubsequentEventsDetailsLineItems" xlink:to="esea_DebtInstrumentNumberOfSemiAnnualPayments" order="7" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="esea_Note20SubsequentEventsDetailsLineItems" xlink:to="us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment" order="8" xbrldt:closed="true"/> 
  </definitionLink>     
  <definitionLink xlink:type="extended" xlink:role="http://www.euroseas.gr/role/DocumentAndEntityInformation"> 
    <loc xlink:type="locator" xlink:label="dei_DocumentInformationLineItems" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_DocumentInformationLineItems"/>   
    <loc xlink:type="locator" xlink:label="dei_EntityRegistrantName" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityRegistrantName"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityRegistrantName" order="0" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="dei_TradingSymbol" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_TradingSymbol"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_TradingSymbol" order="1" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="dei_DocumentType" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_DocumentType"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_DocumentType" order="2" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="dei_CurrentFiscalYearEndDate" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_CurrentFiscalYearEndDate"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_CurrentFiscalYearEndDate" order="3" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="dei_EntityCommonStockSharesOutstanding" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityCommonStockSharesOutstanding"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityCommonStockSharesOutstanding" order="4" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="dei_EntityPublicFloat" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityPublicFloat"/> 
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityPublicFloat" order="5" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="dei_AmendmentFlag" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_AmendmentFlag"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_AmendmentFlag" order="6" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="dei_AmendmentDescription" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_AmendmentDescription"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_AmendmentDescription" order="7" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="dei_EntityCentralIndexKey" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityCentralIndexKey"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityCentralIndexKey" order="8" xbrldt:closed="true"/>    
    <loc xlink:type="locator" xlink:label="dei_EntityCurrentReportingStatus" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityCurrentReportingStatus"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityCurrentReportingStatus" order="9" xbrldt:closed="true"/> 
    <loc xlink:type="locator" xlink:label="dei_EntityVoluntaryFilers" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityVoluntaryFilers"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityVoluntaryFilers" order="10" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="dei_EntityFilerCategory" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityFilerCategory"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityFilerCategory" order="11" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="dei_EntityWellKnownSeasonedIssuer" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_EntityWellKnownSeasonedIssuer"/>     
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_EntityWellKnownSeasonedIssuer" order="12" xbrldt:closed="true"/>   
    <loc xlink:type="locator" xlink:label="dei_DocumentPeriodEndDate" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_DocumentPeriodEndDate"/>    
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_DocumentPeriodEndDate" order="13" xbrldt:closed="true"/>     
    <loc xlink:type="locator" xlink:label="dei_DocumentFiscalYearFocus" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_DocumentFiscalYearFocus"/>   
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_DocumentFiscalYearFocus" order="14" xbrldt:closed="true"/>  
    <loc xlink:type="locator" xlink:label="dei_DocumentFiscalPeriodFocus" xlink:href="http://xbrl.sec.gov/dei/2014/dei-2014-01-31.xsd#dei_DocumentFiscalPeriodFocus"/>  
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems" xlink:to="dei_DocumentFiscalPeriodFocus" order="15" xbrldt:closed="true"/>    
  </definitionLink>     
</linkbase>  
